Overview

  • Product name
  • Description
    Rabbit polyclonal to Apc4
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to N terminal region of Human Apc4.

  • Positive control
    • Human spleen and uterus tissues; Mouse liver tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 92 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the APC4 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANAPC 4 antibody
    • anapc4 antibody
    • Anaphase Promoting Complex Subunit 4 antibody
    • Anaphase-promoting complex subunit 4 antibody
    • APC 4 antibody
    • APC Complex Protein CUT20 antibody
    • APC4 antibody
    • APC4_HUMAN antibody
    • CUT20 antibody
    • Cyclosome subunit 4 antibody
    • LID1 antibody
    see all

Anti-Apc4 antibody images

  • ab113364, at 5µg/ml, staining Apc4 in Formalin-fixed, Paraffin-embedded Human Spleen tissue by Immunohistochemistry.
  • ab113364, at 5µg/ml, staining Apc4 in Formalin-fixed, Paraffin-embedded Human uterus tissue by Immunohistochemistry.
  • Lane 1 : Anti-Apc4 antibody (ab113364) at 1 µg/ml
    Lane 2 : Anti-Apc4 antibody (ab113364) at 2 µg/ml

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse liver tissue lysate


    Predicted band size : 92 kDa

References for Anti-Apc4 antibody (ab113364)

ab113364 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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