Overview

  • Product nameAnti-Apc4 antibody
    See all Apc4 primary antibodies
  • Description
    Rabbit polyclonal to Apc4
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 758 and 808 of human Apc4 (NP_037499.2).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the APC4 family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANAPC 4 antibody
    • anapc4 antibody
    • Anaphase Promoting Complex Subunit 4 antibody
    • Anaphase-promoting complex subunit 4 antibody
    • APC 4 antibody
    • APC Complex Protein CUT20 antibody
    • APC4 antibody
    • APC4_HUMAN antibody
    • CUT20 antibody
    • Cyclosome subunit 4 antibody
    • LID1 antibody
    see all

Anti-Apc4 antibody images

  • All lanes : Anti-Apc4 antibody (ab72149) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 92 kDa
    Observed band size : 92 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human Apc4 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72149 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

References for Anti-Apc4 antibody (ab72149)

This product has been referenced in:
  • Ma HT & Poon RY Orderly Inactivation of the Key Checkpoint Protein Mitotic Arrest Deficient 2 (MAD2) during Mitotic Progression. J Biol Chem 286:13052-9 (2011). WB ; Human . Read more (PubMed: 21335556) »
  • Mo M  et al. Cell cycle deregulation by a poxvirus partial mimic of anaphase-promoting complex subunit 11. Proc Natl Acad Sci U S A 106:19527-32 (2009). WB ; Human . Read more (PubMed: 19887645) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"