Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab4170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ICC 1/100.

Target

  • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the APC5 family.
    Contains 4 TPR repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2510006G12Rik antibody
    • AA408751 antibody
    • AA536819 antibody
    • AA986414 antibody
    • ANAPC 5 antibody
    • ANAPC5 antibody
    • Anaphase promoting complex subunit 5 antibody
    • Anaphase-promoting complex subunit 5 antibody
    • Apc 5 antibody
    • APC5 antibody
    • APC5_HUMAN antibody
    • Cyclosome subunit 5 antibody
    • PDL 108 antibody
    • PDL108 antibody
    • Periodontal ligament fibroblast protein antibody
    • RMC1 antibody
    see all

Anti-Apc5 antibody images

  • Metaphase HeLa cells labelled with APC5 (ab4170) and counterstained with DAPI.
    The antibody shows foci of APC5 localised to the centrosomes. 100x magnification.

    Panel 1: DNA stained with DAPI
    Panel 2: Cells stained with ab4170 (1/100 dilution)
    Panel 3: DAPI staining is shown in red, ab4170 staining is shown in green

    Metaphase HeLa cells labelled with APC5 (ab4170) and counterstained with DAPI.
    The antibody shows foci of APC5 localised to the centrosomes. 100x magnification.

    Panel 1: DNA stained with DAPI
    Panel 2: Cells stained with ab4170 (1/100 dilution)
    Panel 3: DAPI staining is shown in red, ab4170 staining is shown in green

References for Anti-Apc5 antibody (ab4170)

This product has been referenced in:
  • Tegha-Dunghu J  et al. EML3 is a nuclear microtubule-binding protein required for the correct alignment of chromosomes in metaphase. J Cell Sci 121:1718-26 (2008). ICC/IF ; Human . Read more (PubMed: 18445686) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Specification Hela
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Aug 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cell (HeLa, SK-N-SH and Indian muntjac)
Specification HeLa, SK-N-SH and Indian muntjac
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Oct 31 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"