Overview

  • Product nameAnti-Apc5 antibody
    See all Apc5 primary antibodies
  • Description
    Rabbit polyclonal to Apc5
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide from a region between residue 705 and the C terminus (residue 755) of human Apc5 (NP_057321.2)

  • Positive control
    • Whole cell lysate from Hela, 293T and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the APC5 family.
    Contains 4 TPR repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2510006G12Rik antibody
    • AA408751 antibody
    • AA536819 antibody
    • AA986414 antibody
    • ANAPC 5 antibody
    • ANAPC5 antibody
    • Anaphase promoting complex subunit 5 antibody
    • Anaphase-promoting complex subunit 5 antibody
    • Apc 5 antibody
    • APC5 antibody
    • APC5_HUMAN antibody
    • Cyclosome subunit 5 antibody
    • PDL 108 antibody
    • PDL108 antibody
    • Periodontal ligament fibroblast protein antibody
    • RMC1 antibody
    see all

Anti-Apc5 antibody images

  • All lanes : Anti-Apc5 antibody (ab72516) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 85 kDa
    Observed band size : 85 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation/ Western Blot of Apc5.
    Lane 1: ab72516 at 3µg/mg Hela whole cell lysate (1mg for IP, 20% of IP loaded).
    Lane 2: Control IgG.
    ab72516 at 1µg/ml for WB.
    Chemiluminescence with an exposure time of 1 minute.

References for Anti-Apc5 antibody (ab72516)

This product has been referenced in:
  • Dai L  et al. Dose-dependent proteomic analysis of glioblastoma cancer stem cells upon treatment with ?-secretase inhibitor. Proteomics : (2011). WB ; Human . Read more (PubMed: 21932445) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"