Overview

  • Product name
  • Description
    Rabbit polyclonal to Apc6
  • Specificity
    Detects endogenous levels of total Apc6 protein
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic non-phosphopeptide (Human) from around the phosphorylation site of serine 560 (IISPPP)

  • Positive control
    • Breast carcinoma tissue. HUVEC cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab61189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
IHC-P 1/50 - 1/100.
ELISA 1/5000.

Target

  • Function
    Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the APC6/CDC16 family.
    Contains 7 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
  • Cellular localization
    Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANAPC6 antibody
    • Anaphase promoting complex subunit 6 antibody
    • Anaphase-promoting complex subunit 6 antibody
    • Apc 6 antibody
    • APC6 antibody
    • CDC 16 antibody
    • CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
    • Cdc16 antibody
    • CDC16 homolog antibody
    • CDC16 protein antibody
    • CDC16_HUMAN antibody
    • CDC16Hs antibody
    • Cell division cycle 16 antibody
    • Cell division cycle 16 homolog antibody
    • Cell division cycle protein 16 homolog antibody
    • CUT9 antibody
    • Cyclosome subunit 6 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab61189 at 1/50 dilution. Samples were treated -/+ peptide.
  • All lanes : Anti-Apc6 antibody (ab61189) at 1/500 dilution

    Lane 1 : HUVEC extract
    Lane 2 : HUVEC extract with peptide


    Predicted band size : 72 kDa
    Observed band size : 72 kDa

References

This product has been referenced in:
  • Holt JE  et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010). WB ; Mouse . Read more (PubMed: 20223764) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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