Overview

  • Product nameAnti-Apc6 antibody
    See all Apc6 primary antibodies
  • Description
    Rabbit polyclonal to Apc6
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Guinea pig, Chimpanzee, Lizard, Rhesus monkey, Gorilla, Opossum
  • Immunogen

    Synthetic peptide corresponding to a region between residues 175 and 225 of human Apc6, NP_003894.3.

  • Positive control
    • Whole cell lysate from Hela and NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72136 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 72, 90 kDa (predicted molecular weight: 72 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the APC6/CDC16 family.
    Contains 7 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANAPC6 antibody
    • Anaphase promoting complex subunit 6 antibody
    • Anaphase-promoting complex subunit 6 antibody
    • Apc 6 antibody
    • APC6 antibody
    • CDC 16 antibody
    • CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
    • Cdc16 antibody
    • CDC16 homolog antibody
    • CDC16 protein antibody
    • CDC16_HUMAN antibody
    • CDC16Hs antibody
    • Cell division cycle 16 antibody
    • Cell division cycle 16 homolog antibody
    • Cell division cycle protein 16 homolog antibody
    • CUT9 antibody
    • Cyclosome subunit 6 antibody
    see all

Anti-Apc6 antibody images

  • All lanes : Anti-Apc6 antibody (ab72136) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 15 µg
    Lane 2 : Whole cell lysate from Hela cells at 5 µg
    Lane 3 : Whole cell lysate from Hela cells at 1.5 µg
    Lane 4 : Whole cell lysate from Hela cells at 0.5 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg


    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.
  • Immunoprecipitation/ Western Blot of Apc6.
    Lane 1: ab72136 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.

    ab72136 at 0.1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection:Chemiluminescence with an exposure time of 30 seconds.

References for Anti-Apc6 antibody (ab72136)

ab72136 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"