Recombinant
RabMAb

Anti-Apc6 antibody [EPR11168] (ab169536)

Overview

  • Product name
    Anti-Apc6 antibody [EPR11168]
    See all Apc6 primary antibodies
  • Description
    Rabbit monoclonal [EPR11168] to Apc6
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Apc6 (UniProt Q13042).

  • Positive control
    • HT-1080, HepG2 and HeLa cell lysates; Human breast carcinoma, gastric carcinoma and kidney tissues; HeLa cells; Immunoprecipitation pellet from HepG2 cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 71 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the APC6/CDC16 family.
      Contains 7 TPR repeats.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
    • Cellular localization
      Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANAPC6 antibody
      • Anaphase promoting complex subunit 6 antibody
      • Anaphase-promoting complex subunit 6 antibody
      • Apc 6 antibody
      • APC6 antibody
      • CDC 16 antibody
      • CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
      • Cdc16 antibody
      • CDC16 homolog antibody
      • CDC16 protein antibody
      • CDC16_HUMAN antibody
      • CDC16Hs antibody
      • Cell division cycle 16 antibody
      • Cell division cycle 16 homolog antibody
      • Cell division cycle protein 16 homolog antibody
      • CUT9 antibody
      • Cyclosome subunit 6 antibody
      see all

    Images

    • All lanes : Anti-Apc6 antibody [EPR11168] (ab169536) at 1/1000 dilution

      Lane 1 : HT-1080 cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 71 kDa

    • Anti-Apc6 antibody [EPR11168] (ab169536) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate

      Secondary
      HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

      Predicted band size: 71 kDa

    • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Apc6 with ab169536 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Apc6 with ab169536 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Apc6 with ab169536 at 1/50 dilution.
    • Immunofluorescent analysis of HeLa cells labeling Apc6 with ab169536 at 1/100 dilution.

    References

    ab169536 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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