Anti-Apc6 (phospho S560) antibody (ab61075)

Overview

  • Product nameAnti-Apc6 (phospho S560) antibody
    See all Apc6 primary antibodies
  • Description
    Rabbit polyclonal to Apc6 (phospho S560)
  • SpecificityDetects endogenous levels of CDC16/APC6 only when phosphorylated at serine 560.
  • Tested applicationsSuitable for: ELISA, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized phosphopeptide derived from human CDC16/APC6 around the phosphorylation site of serine 560 (IISpPP).

  • Positive control
    • Human brain tissue; extracts from HuvEc cells; HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 72 kDa.

Target

  • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the APC6/CDC16 family.
    Contains 7 TPR repeats.
  • Post-translational
    modifications
    Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
  • Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANAPC6 antibody
    • Anaphase promoting complex subunit 6 antibody
    • Anaphase-promoting complex subunit 6 antibody
    • Apc 6 antibody
    • APC6 antibody
    • CDC 16 antibody
    • CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
    • Cdc16 antibody
    • CDC16 homolog antibody
    • CDC16 protein antibody
    • CDC16_HUMAN antibody
    • CDC16Hs antibody
    • Cell division cycle 16 antibody
    • Cell division cycle 16 homolog antibody
    • Cell division cycle protein 16 homolog antibody
    • CUT9 antibody
    • Cyclosome subunit 6 antibody
    see all

Anti-Apc6 (phospho S560) antibody images

  • All lanes : Anti-Apc6 (phospho S560) antibody (ab61075) at 1/500 dilution

    Lane 1 : extracts from HuvEc cells
    Lane 2 : extracts from HuvEc cells with phospho-peptide


    Predicted band size : 72 kDa
    Observed band size : 72 kDa
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61075 at 1/50 dilution with or without phospho peptide.
  • Immunofluorescence analysis of HeLa cells, using ab61075 at 1/500 dilution with or without phospho peptide.

References for Anti-Apc6 (phospho S560) antibody (ab61075)

ab61075 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"