Anti-Apc6 (phospho T580) antibody (ab12282)

Overview

  • Product name
    Anti-Apc6 (phospho T580) antibody
    See all Apc6 primary antibodies
  • Description
    Rabbit polyclonal to Apc6 (phospho T580)
  • Specificity
    ab12282 is specific for the phosphorylated form of APC6 at the T580 residue. MInimal reactivity is expected with the non-phosphorylated form of the protein.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: CG-ETGLTpPLETS conjugated to KLH, corresponding to Internal sequence amino acids 575-588 of Human Apc6.

Properties

Applications

Our Abpromise guarantee covers the use of ab12282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes
    IF: Use at an assay dependent dilution.
    WB: 1/500 - 1/3000. Expect bands of approximately 72kDa, 71.5kDa or 65.9 kDa depending on the isoform (predicted molecular weight: 72 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the APC6/CDC16 family.
      Contains 7 TPR repeats.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
    • Cellular localization
      Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANAPC6 antibody
      • Anaphase promoting complex subunit 6 antibody
      • Anaphase-promoting complex subunit 6 antibody
      • Apc 6 antibody
      • APC6 antibody
      • CDC 16 antibody
      • CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
      • Cdc16 antibody
      • CDC16 homolog antibody
      • CDC16 protein antibody
      • CDC16_HUMAN antibody
      • CDC16Hs antibody
      • Cell division cycle 16 antibody
      • Cell division cycle 16 homolog antibody
      • Cell division cycle protein 16 homolog antibody
      • CUT9 antibody
      • Cyclosome subunit 6 antibody
      see all

    Images

    • All lanes : Anti-Apc6 (phospho T580) antibody (ab12282) at 1/1000 dilution

      Lane 1 : Nocodazole treated HeLa whole cell lysate
      Lane 2 : Asynchronous HeLa whole cell lysate

      Lysates/proteins at 35 µg per lane.

      Secondary
      Goat anti-Rabbit IgG at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 72 kDa
      Observed band size : 72 kDa

    References

    ab12282 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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