Anti-Apc6 (phospho T580) antibody (ab12282)

Overview

  • Product nameAnti-Apc6 (phospho T580) antibody
    See all Apc6 primary antibodies
  • Description
    Rabbit polyclonal to Apc6 (phospho T580)
  • Specificityab12282 is specific for the phosphorylated form of APC6 at the T580 residue. MInimal reactivity is expected with the non-phosphorylated form of the protein.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: CG-ETGLTpPLETS conjugated to KLH, corresponding to Internal sequence amino acids 575-588 of Human Apc6.

Properties

Applications

Our Abpromise guarantee covers the use of ab12282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesIF: Use at an assay dependent dilution.
    WB: 1/500 - 1/3000. Expect bands of approximately 72kDa, 71.5kDa or 65.9 kDa depending on the isoform (predicted molecular weight: 72 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
    • PathwayProtein modification; protein ubiquitination.
    • Sequence similaritiesBelongs to the APC6/CDC16 family.
      Contains 7 TPR repeats.
    • Post-translational
      modifications
      Phosphorylated. Phosphorylation on Ser-560 occurs specifically during mitosis.
    • Cellular localizationCytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANAPC6 antibody
      • Anaphase promoting complex subunit 6 antibody
      • Anaphase-promoting complex subunit 6 antibody
      • Apc 6 antibody
      • APC6 antibody
      • CDC 16 antibody
      • CDC16 (cell division cycle 16 S. cerevisiae homolog) antibody
      • Cdc16 antibody
      • CDC16 homolog antibody
      • CDC16 protein antibody
      • CDC16_HUMAN antibody
      • CDC16Hs antibody
      • Cell division cycle 16 antibody
      • Cell division cycle 16 homolog antibody
      • Cell division cycle protein 16 homolog antibody
      • CUT9 antibody
      • Cyclosome subunit 6 antibody
      see all

    Anti-Apc6 (phospho T580) antibody images

    • All lanes : Anti-Apc6 (phospho T580) antibody (ab12282) at 1/1000 dilution

      Lane 1 : Nocodazole treated HeLa whole cell lysate
      Lane 2 : Asynchronous HeLa whole cell lysate

      Lysates/proteins at 35 µg per lane.

      Secondary
      Goat anti-Rabbit IgG at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 72 kDa
      Observed band size : 72 kDa

    References for Anti-Apc6 (phospho T580) antibody (ab12282)

    ab12282 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab12282.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"