Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)


  • Product nameAnti-APE1 antibody [13B8E5C2] - ChIP Grade
    See all APE1 primary antibodies
  • Description
    Mouse monoclonal [13B8E5C2] to APE1 - ChIP Grade
  • SpecificityThis antibody is specific to the human APE/ref-1 protein.
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IP, WB, ELISA, ICC, IHC-P, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length native protein (purified) (Human).

  • General notesAPE appears to form a unique link between the DNA base excision pathway, oxidative signalling, trancription regulation, cancer and cell-cycle control.



Our Abpromise guarantee covers the use of ab194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/5000.
IP Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 35.6 kDa.
ELISA 1/5000.
ICC Use at an assay dependent concentration.
IHC-P 1/5000.
ChIP Use at an assay dependent concentration.


  • FunctionMultifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.
  • Sequence similaritiesBelongs to the DNA repair enzymes AP/ExoA family.
  • DomainThe N-terminus contains the redox activity while the C-terminus exerts the DNA AP-endodeoxyribonuclease activity; both function are independent in their actions. An unconventional mitochondrial targeting sequence (MTS) is harbored within the C-terminus, that appears to be masked by the N-terminal sequence containing the nuclear localization signal (NLS), that probably blocks the interaction between the MTS and Tom proteins.
  • Post-translational
    Phosphorylated. Phosphorylation by kinase PKC or casein kinase CK2 results in enhanced redox activity that stimulates binding of the FOS/JUN AP-1 complex to its cognate binding site. AP-endodeoxyribonuclease activity is not affected by CK2-mediated phosphorylation. Phosphorylation of Thr-233 by CDK5 reduces AP-endodeoxyribonuclease activity resulting in accumulation of DNA damage and contributing to neuronal death.
    Acetylated on Lys-6 and Lys-7. Acetylation is increased by the transcriptional coactivator EP300 acetyltransferase, genotoxic agents like H(2)O(2) and methyl methanesulfonate (MMS). Acetylation increases its binding affinity to the negative calcium response element (nCaRE) DNA promoter. The acetylated form induces a stronger binding of YBX1 to the Y-box sequence in the MDR1 promoter than the unacetylated form. Deacetylated on lysines. Lys-6 and Lys-7 are deacetylated by SIRT1.
    Cleaved at Lys-31 by granzyme A to create the mitochondrial form; leading in reduction of binding to DNA, AP endodeoxynuclease activity, redox activation of transcription factors and to enhanced cell death. Cleaved by granzyme K; leading to intracellular ROS accumulation and enhanced cell death after oxidative stress.
    Cys-65 and Cys-93 are nitrosylated in response to nitric oxide (NO) and lead to the exposure of the nuclear export signal (NES).
    Ubiquitinated by MDM2; leading to translocation to the cytoplasm and proteasomal degradation.
  • Cellular localizationMitochondrion. The cleaved APEX2 is only detected in mitochondria (By similarity). Translocation from the cytoplasm to the mitochondria is mediated by ROS signaling and cleavage mediated by granzyme A. Tom20-dependent translocated mitochondrial APEX1 level is significantly increased after genotoxic stress and Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Detected in the cytoplasm of B-cells stimulated to switch (By similarity). Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310 regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized predominantly in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • AP endonuclease 1 antibody
    • AP endonuclease class I antibody
    • AP lyase antibody
    • APE 1 antibody
    • APE antibody
    • APE-1 antibody
    • APEN antibody
    • APEX 1 antibody
    • APEX antibody
    • APEX nuclease (multifunctional DNA repair enzyme) 1 antibody
    • Apex nuclease 1 antibody
    • APEX nuclease antibody
    • APEX1 antibody
    • APEX1_HUMAN antibody
    • Apurinic endonuclease antibody
    • Apurinic-apyrimidinic endonuclease 1 antibody
    • Apurinic/apyrimidinic (abasic) endonuclease antibody
    • Apurinic/apyrimidinic endonuclease 1 antibody
    • Apurinic/apyrimidinic exonuclease antibody
    • APX antibody
    • BAP1 antibody
    • Deoxyribonuclease (apurinic or apyrimidinic) antibody
    • DNA (apurinic or apyrimidinic site) lyase antibody
    • DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody
    • EC antibody
    • HAP 1 antibody
    • HAP1 antibody
    • Human Apurinic endonuclease 1 antibody
    • MGC139790 antibody
    • Multifunctional DNA repair enzyme antibody
    • Redox factor 1 antibody
    • Redox factor-1 antibody
    • REF 1 antibody
    • REF 1 protein antibody
    • REF-1 antibody
    • REF1 antibody
    • REF1 protein antibody
    see all

Anti-APE1 antibody [13B8E5C2] - ChIP Grade images

  • ab194 staining cultured human HeLa cells by ICC/IF.  Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 20°C.  A Cy3® conjugated donkey anti-mouse antibody diluted was used as the secondary.

    See Abreview

  • Ab194 staining Human normal placenta. Staining is localized to the nucleus.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-APE1 antibody [13B8E5C2] - ChIP Grade (ab194)

This product has been referenced in:
  • Sassa A  et al. Xeroderma Pigmentosum Group A Suppresses Mutagenesis Caused by Clustered Oxidative DNA Adducts in the Human Genome. PLoS One 10:e0142218 (2015). WB ; Human . Read more (PubMed: 26559182) »
  • Xu B  et al. The changes of 8-OHdG, hOGG1, APE1 and Pol ß in lenses of patients with age-related cataract. Curr Eye Res 40:378-85 (2015). WB . Read more (PubMed: 24911554) »

See all 25 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Human Cell (Human primary fibroblasts)
Specification Human primary fibroblasts
Permeabilization Yes - 0.2% Triton-X100
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 13 2013

Vials of this product may appear pink in colour as it is produced in ascites and not purified. Therefore this may give some discolouration as the product contains serum proteins.

The exact epitope recognized by this antibody is not known, as it was raised to the full-length human protein and the epitope has not been mapped.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Purified protein (purified APE1 protein)
Specification purified APE1 protein
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Type Sandwich (Detection)

Ms. Yu ran Lee

Verified customer

Submitted Dec 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (APE1 protein)
Total protein in input 0.1 µg
Specification APE1 protein
Immuno-precipitation step Protein A/G

Ms. Yu ran Lee

Verified customer

Submitted Dec 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Liver, heart, kidney)
Loading amount 40 µg
Specification Liver, heart, kidney
Gel Running Conditions Reduced Denaturing (12% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Ms. Yu ran Lee

Verified customer

Submitted Dec 10 2012

Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guaran...

Read More
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HeLa cells)
Total protein in input 200 µg
Specification HeLa cells
Immuno-precipitation step Protein A/G

Abcam user community

Verified customer

Submitted Mar 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa WCE)
Loading amount 25 µg
Specification HeLa WCE
Gel Running Conditions Reduced Denaturing (10% Tris-Gly)
Blocking step LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C

Dr. Svetlana Khoronenkova

Verified customer

Submitted Aug 12 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (1 EBNA, 2 HeLa)
Loading amount 15 µg
Specification 1 EBNA, 2 HeLa
Gel Running Conditions Reduced Denaturing
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2%

Abcam user community

Verified customer

Submitted Dec 16 2008