Overview

  • Product name
  • Description
    Rabbit polyclonal to Apelin
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide from the C terminal region of Human Apelin.

  • Positive control
    • U87, MCF7, HeLa, MM453 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab125213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 9 kDa. The detection limit for ab125213 is approximately 1ng/lane under non-reducing and reducing conditions.

Target

  • Function
    Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior.
  • Tissue specificity
    Expressed in the brain with highest levels in the frontal cortex, thalamus, hypothalamus and midbrain. Secreted by the mammary gland into the colostrum and the milk.
  • Sequence similarities
    Belongs to the apelin family.
  • Post-translational
    modifications
    Several active peptides may be produced by proteolytic processing of the peptide precursor.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGTRL1 ligand antibody
    • APEL antibody
    • APEL_HUMAN antibody
    • Apelin-13 antibody
    • APJ endogenous ligand antibody
    • Apln antibody
    • XNPEP2 antibody
    see all

Anti-Apelin antibody images

  • All lanes : Anti-Apelin antibody (ab125213) at 0.5 µg/ml

    Lane 1 : U87 Cell lysate
    Lane 2 : MCF-7 Cell Lysate
    Lane 3 : HELA Cell Lysate
    Lane 4 : MM453 Cell lysate

    Lysates/proteins at 40 µg per lane.


    Predicted band size : 9 kDa

References for Anti-Apelin antibody (ab125213)

This product has been referenced in:
  • Chen L  et al. Apelin Protects Primary Rat Retinal Pericytes from Chemical Hypoxia-Induced Apoptosis. J Ophthalmol 2015:186946 (2015). WB ; Rat . Read more (PubMed: 26491547) »

See 1 Publication for this product

Product Wall

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Lung section)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
Permeabilization
No
Specification
Lung section
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Amreen Mughal

Verified customer

Submitted Mar 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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