Overview

  • Product nameAnti-APEX2 antibody
    See all APEX2 primary antibodies
  • Description
    Rabbit polyclonal to APEX2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptides of human APEXL2.

  • Positive control
    • IHC-P: FFPE human cerebral cortex.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 57 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionFunction as a weak apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Displays also double-stranded DNA 3'-5' exonuclease, 3'-phosphodiesterase activities. Shows robust 3'-5' exonuclease activity on 3'-recessed heteroduplex DNA and is able to remove mismatched nucleotides preferentially. Shows fairly strong 3'-phosphodiesterase activity involved in the removal of 3'-damaged termini formed in DNA by oxidative agents. In the nucleus functions in the PCNA-dependent BER pathway. Required for somatic hypermutation (SHM) and DNA cleavage step of class switch recombination (CSR) of immunoglobulin genes. Required for proper cell cycle progression during proliferation of peripheral lymphocytes.
  • Tissue specificityHighly expressed in brain and kidney. Weakly expressed in the fetal brain.
  • Sequence similaritiesBelongs to the DNA repair enzymes AP/ExoA family.
  • Cellular localizationNucleus. Cytoplasm. Mitochondrion. Together with PCNA, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • AP endonuclease 2 antibody
    • AP endonuclease XTH2 antibody
    • APE 2 antibody
    • APE2 antibody
    • APEX 2 antibody
    • APEX L2 antibody
    • APEX nuclease (apurinic/apyrimidinic endonuclease) 2 antibody
    • APEX nuclease 2 antibody
    • APEX nuclease like 2 antibody
    • APEX nuclease-like 2 antibody
    • APEX2 antibody
    • APEX2_HUMAN antibody
    • APEXL 2 antibody
    • APEXL2 antibody
    • Apurinic apyrimidinic endonuclease 2 antibody
    • Apurinic-apyrimidinic endonuclease 2 antibody
    • Apurinic/apyrimidinic endonuclease 2 antibody
    • Apurinic/apyrimidinic endonuclease like 2 antibody
    • C430040P13Rik antibody
    • DNA (apurinic or apyrimidinic site) lyase 2 antibody
    • DNA apurinic or apyrimidinic site lyase 2 antibody
    • DNA-(apurinic or apyrimidinic site) lyase 2 antibody
    • EC 4.2.99.18 antibody
    • OTTHUMP00000023390 antibody
    • OTTHUMP00000061908 antibody
    • XTH 2 antibody
    • XTH2 antibody
    see all

Anti-APEX2 antibody images

  • IHC image of APEX2 staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13691, 1/1000, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 57 kDa

    Western blot analysis of APEXL2 in HeLa cell lysates using ab13691 at a dilution of 1:500.

    Western blot analysis of APEXL2 in HeLa cell lysates using ab13691 at a dilution of 1:500.

References for Anti-APEX2 antibody (ab13691)

This product has been referenced in:
  • Karkhanis V  et al. Protein Arginine Methyltransferase 7 Regulates Cellular Response to DNA Damage by Methylating Promoter Histones H2A and H4 of the Polymerase d Catalytic Subunit Gene, POLD1. J Biol Chem 287:29801-14 (2012). WB ; Human . Read more (PubMed: 22761421) »
  • Akbari M  et al. Mitochondrial base excision repair of uracil and AP sites takes place by single-nucleotide insertion and long-patch DNA synthesis. DNA Repair (Amst) 7:605-16 (2008). WB ; Human . Read more (PubMed: 18295553) »

See all 2 Publications for this product

Product Wall

The antibody was made against two synthetic peptide sequences (rabbit was injected with a mixture of these peptides): GenBank Accession no. NM-014481. 1. amino acids 122-140 2. amino acids 438-455. If you have any additional questions, please cont...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"