Overview

  • Product name
  • Description
    Rabbit polyclonal to Apg10
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Apg10.

    (Peptide available as ab90636.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: DU 145; Raji.

Properties

Applications

Our Abpromise guarantee covers the use of ab79316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Function
    E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
  • Sequence similarities
    Belongs to the ATG10 family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apg 10 antibody
    • APG 10L antibody
    • APG10 antibody
    • APG10 autophagy 10 like antibody
    • APG10 like antibody
    • APG10, S. cerevisiae, homolog of antibody
    • APG10-like antibody
    • APG10L antibody
    • ATG 10 antibody
    • ATG10 antibody
    • ATG10 autophagy related 10 homolog (S. cerevisiae) antibody
    • ATG10 autophagy related 10 homolog antibody
    • ATG10_HUMAN antibody
    • autophagy 10, S. cerevisiae, homolog of antibody
    • Autophagy related protein 10 antibody
    • Autophagy-related protein 10 antibody
    • DKFZP586I0418 antibody
    • FLJ13954 antibody
    • pp12616 antibody
    • Ubiquitin-like-conjugating enzyme ATG10 antibody
    see all

Images

  • All lanes : Anti-Apg10 antibody (ab79316) at 1 µg/ml

    Lane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 34 kDa,37 kDa,42 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References

ab79316 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab79316.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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