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Rabbit polyclonal to Apg10
Predicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Apg10.
(Peptide available as
This antibody gave a positive signal in the following whole cell lysates: DU 145; Raji.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
Belongs to the ATG10 family.
Information by UniProt
Apg 10 antibody
APG 10L antibody
Western blot - Anti-Apg10 antibody (ab79316)
All lanes : Anti-Apg10 antibody (ab79316) at 1 µg/ml Lane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa Additional bands at: 34 kDa, 37 kDa, 42 kDa. We are unsure as to the identity of these extra bands. Exposure time: 3 minutes
has not yet been referenced specifically in any publications.
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