Overview

  • Product nameAnti-Apg12 antibody
    See all Apg12 primary antibodies
  • Description
    Rabbit polyclonal to Apg12
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein (S. cerevisiae).

  • Positive control
    • Most yeast cell lysates can be used as a positive control without induction or stimulation.

Properties

Applications

Our Abpromise guarantee covers the use of ab4752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/1000 - 1/5000.
    WB: 1/2000. Detects a band of approximately 22.1 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.
  • Target

    • FunctionUbiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
    • Tissue specificityUbiquitous.
    • Sequence similaritiesBelongs to the ATG12 family.
    • DomainShares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Alternative names
      • APG12-like antibody
      • APG12L antibody
      • ATG12 antibody
      • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
      • ATG12 autophagy related 12 homolog antibody
      • ATG12_HUMAN antibody
      • Autophagy 12 antibody
      • Autophagy-related protein 12 antibody
      • FBR93 antibody
      • HAPG12 antibody
      • Ubiquitin-like protein ATG12 antibody
      see all

    Anti-Apg12 antibody images

    • ab4752 generated by immunization with recombinant yeast APG12 was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1:15,000 dilution of Donkey polyclonal to Goat IgG (HRP) (ab7125). Panel C shows specific reaction with APG12 using a 1:2,000 dilution of ab4752 followed by reaction with a 1:15,000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. Yeast lysate proteins were separated by SDS-PAGE using a 15% gel. This data indicates that anti-APG12 is highly specific and does not cross react with other UBLs. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication.

    References for Anti-Apg12 antibody (ab4752)

    This product has been referenced in:
    • Crews L  et al. Selective Molecular Alterations in the Autophagy Pathway in Patients with Lewy Body Disease and in Models of alpha-Synucleinopathy. PLoS One 5:e9313 (2010). WB ; Human, Mouse . Read more (PubMed: 20174468) »

    See 1 Publication for this product

    Product Wall

    Sorry but there has been no further information on this and we do not know if it will cross react with human.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"