Anti-Apg12 antibody [EPR4799] - Autophagosome Marker (ab109492)

Overview

  • Product nameAnti-Apg12 antibody [EPR4799] - Autophagosome Marker
    See all Apg12 primary antibodies
  • Description
    Rabbit monoclonal [EPR4799] to Apg12 - Autophagosome Marker
  • Tested applicationsSuitable for: ICC, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Apg12.

  • Positive control
    • THP1, HepG2, Raji, and Human fetal kidney lysate; Apg12-transfected 293T lysates; Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-Apg12 antibody (Alexa Fluor® 488) - Autophagosome Marker [EPR4799] (ab185028)

Properties

Applications

Our Abpromise guarantee covers the use of ab109492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/10000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionUbiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the ATG12 family.
  • DomainShares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • APG12-like antibody
    • APG12L antibody
    • ATG12 antibody
    • ATG12 autophagy related 12 homolog (S. cerevisiae) antibody
    • ATG12 autophagy related 12 homolog antibody
    • ATG12_HUMAN antibody
    • Autophagy 12 antibody
    • Autophagy-related protein 12 antibody
    • FBR93 antibody
    • HAPG12 antibody
    • Ubiquitin-like protein ATG12 antibody
    see all

Anti-Apg12 antibody [EPR4799] - Autophagosome Marker images

  • Overlay histogram showing HepG2 cells stained with ab109492 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109492, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • All lanes : Anti-Apg12 antibody [EPR4799] - Autophagosome Marker (ab109492) at 1/1000 dilution

    Lane 1 : THP1 lysate
    Lane 2 : HepG2 lysate
    Lane 3 : Raji lysate
    Lane 4 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 15 kDa
    Lower band shows Apg12.
    Upper band shows Apg12-ATG5 conjugate.
  • All lanes : Anti-Apg12 antibody [EPR4799] - Autophagosome Marker (ab109492) at 1/1000 dilution

    Lane 1 : Apg12-transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 15 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • ab109492 at 1/50 dilution staining Apg12 in paraffin embedded Human colon tissue.

References for Anti-Apg12 antibody [EPR4799] - Autophagosome Marker (ab109492)

ab109492 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (HEK293 cell)
Specification HEK293 cell
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 16°C
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Submitted Apr 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"