Validated using a knockout cell line
Recombinant
RabMAb

Anti-Apg12 antibody [EPR4800] (ab109491)

Overview

  • Product name
    Anti-Apg12 antibody [EPR4800]
    See all Apg12 primary antibodies
  • Description
    Rabbit monoclonal [EPR4800] to Apg12
  • Tested applications
    Suitable for: ICC, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues on the C-terminus in Human Apg12.

  • Positive control
    • Human fetal kidney, HepG2, and Raji lysates; Apg12 transfected 293T lysates; Human breast carcinoma tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/250 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    Images



    • Predicted band size : 15 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Apg12 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HCT116 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109491 observed at 55 kDa. Observed band shows Apg12-ATG5 conjugation. Red - loading control, ab8245, observed at 37 kDa.

      Ab109491 was shown to specifically react with Apg12 in wild-type cells along with additional cross-reactive bands. The band was not seen in Apg12 knockout HAP1 cells. Wild-type and Apg12 knockout samples were subjected to SDS-PAGE.  Ab109491 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Apg12 antibody [EPR4800] (ab109491) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : HepG2 lysate
      Lane 3 : Raji lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa
      Observed band shows Apg12-ATG5 conjugation
    • All lanes : Anti-Apg12 antibody [EPR4800] (ab109491) at 1/1000 dilution

      Lane 1 : Apg12 transfected 293T lysate
      Lane 2 : Non-transfected 293T lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa
    • ab109491, at 1/250 dilution, staining Apg12 in paraffin-embedded Human breast carcinoma by Immunohistochemistry.

    References

    ab109491 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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