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Western blot - apg16 antibody (ab72086)
(enlarge)
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Product Name
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apg16 antibody
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See all apg16 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to apg16
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Immunogen
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A portion of amino acids 25-75 of human apg16 (NP_110430).
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Reacts with
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Hu, Chmp, Mk
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Predicted to react
(species key)
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Ms, Dog
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 - 3 µg/ml. Detects bands of approximately 27, 40, 50 and 68 kDa (predicted molecular weight: 68 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human skeletal muscle lysate.
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Cellular localization
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Cytoplasmic
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Research areas
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Cancer >> Cancer Metabolism >> Autophagy
Cardiovascular >> Heart >> Autophagy >> APG gene products
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ub-like Proteins
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Relevance
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A major contributor to cellular homeostasis is the ability of the cell to strike a balance between the formation and degradation/removal of its cellular components. This process of internal cellular turn-over is called autophagy (self-eating), and is facilitated by a pathway of around 16 interacting proteins in the human. APG3 is a conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. APG16 is essential for autophagy and interacts with ATG5 as part of either the minor or major complexes, respectively composed of 4 sets of ATG12-ATG5 and APG16 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16 (800 kDa). A disruption to the autophagic process is now associated with the progression of several cancers, neurodegenerative disorders and cardiac pathologies.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this apg16 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for apg16 antibody images, references, products related to ab72086 and other tools.
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apg16 antibody images:
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 Western blot - apg16 antibody (ab72086)
All lanes : apg16 antibody (ab72086) at 2 µg/ml
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human skeletal muscle lysate plus immunizing peptide
Predicted band size : 68 kDa
Observed band size : 68 kDa
Additional bands at : 27 kDa (possible isoform),50 kDa (possible isoform).
Note: Multiple isoforms of agp16 can be detected in mouse skeletal muscle lysate using ab72086. The approximately 40 kD band seen in this lysate is also seen in prebleed and is not believed to be relevant.
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Search PubMed (MEDLINE) for references to apg16
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apg16 antibody - more information
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Customer reviews (feedback) regarding apg16 antibody |
Customer FAQs regarding apg16 antibody |
Protocols for apg16 antibody |
Price and availability of products related to apg16 antibody |
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apg16 antibody - related products:
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apg16 antibody - other tools:
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Contact Abcam with a Technical Enquiry about apg16 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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