Overview

  • Product name
  • Description
    Rabbit polyclonal to Apg3
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Apg3.

    (Peptide available as ab66286.)

  • Positive control
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: TE 671, Raji, JEG-3, K562, HeLa - Staurosporine Treated (48hr, 500nM) (data not shown)

Properties

Applications

Our Abpromise guarantee covers the use of ab66287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).

Target

  • Function
    E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
  • Tissue specificity
    Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
  • Sequence similarities
    Belongs to the ATG3 family.
  • Post-translational
    modifications
    Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2610016C12Rik antibody
    • Apg 3 antibody
    • APG3 antibody
    • APG3 autophagy 3 like antibody
    • APG3 like antibody
    • APG3, S. cerevisiae, homolog of antibody
    • APG3-like antibody
    • APG3L antibody
    • Apg3p antibody
    • ATG 3 antibody
    • ATG3 antibody
    • ATG3 autophagy related 3 homolog antibody
    • ATG3 autophagy related 3 homolog (S. cerevisiae) antibody
    • ATG3_HUMAN antibody
    • Autophagy 3, S. cerevisiae, homolog of antibody
    • Autophagy Apg3p/Aut1p like antibody
    • autophagy related 3 antibody
    • Autophagy related protein 3 antibody
    • Autophagy-related protein 3 antibody
    • DKFZp564M1178 antibody
    • FLJ22125 antibody
    • hApg3 antibody
    • MGC15201 antibody
    • OTTHUMP00000214547 antibody
    • OTTHUMP00000214548 antibody
    • PC3 96 antibody
    • Protein PC3-96 antibody
    • Ubiquitin-like-conjugating enzyme ATG3 antibody
    see all

Images

  • All lanes : Anti-Apg3 antibody (ab66287) at 1 µg/ml

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : Raji whole cell lysate (ab30124)
    Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab66287 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66287, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) MCF-7 cells at 5µg/ml.

References

This product has been referenced in:
  • Huang Y  et al. Phospho-?Np63a-dependent regulation of autophagic signaling through transcription and micro-RNA modulation. Cell Cycle 11:1247-59 (2012). Read more (PubMed: 22356768) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66287.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up