Overview

  • Product nameAnti-Apg3 antibody [EPR4801]
    See all Apg3 primary antibodies
  • Description
    Rabbit monoclonal [EPR4801] to Apg3
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Apg3 (N terminal).

  • Positive control
    • K562, HL-60, HeLa or Jurkat cell lysate. Human muscle tissue HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Apg3 antibody (Alexa Fluor® 488) [EPR4801] (ab201518)

    Anti-Apg3 antibody (Alexa Fluor® 555) [EPR4801] (ab202871)

    Anti-Apg3 antibody (HRP) [EPR4801] (ab201752)

Properties

Applications

Our Abpromise guarantee covers the use of ab108251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionE2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE). PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
    • Tissue specificityWidely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
    • Sequence similaritiesBelongs to the ATG3 family.
    • Post-translational
      modifications
      Conjugated to ATG12 at Lys-243. ATG12-conjugation plays a role in regulation of mitochondrial homeostasis and cell death, while it is not involved in PE-conjugation to ATG8-like proteins and autophagy.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2610016C12Rik antibody
      • Apg 3 antibody
      • APG3 antibody
      • APG3 autophagy 3 like antibody
      • APG3 like antibody
      • APG3, S. cerevisiae, homolog of antibody
      • APG3-like antibody
      • APG3L antibody
      • Apg3p antibody
      • ATG 3 antibody
      • ATG3 antibody
      • ATG3 autophagy related 3 homolog antibody
      • ATG3 autophagy related 3 homolog (S. cerevisiae) antibody
      • ATG3_HUMAN antibody
      • Autophagy 3, S. cerevisiae, homolog of antibody
      • Autophagy Apg3p/Aut1p like antibody
      • autophagy related 3 antibody
      • Autophagy related protein 3 antibody
      • Autophagy-related protein 3 antibody
      • DKFZp564M1178 antibody
      • FLJ22125 antibody
      • hApg3 antibody
      • MGC15201 antibody
      • OTTHUMP00000214547 antibody
      • OTTHUMP00000214548 antibody
      • PC3 96 antibody
      • Protein PC3-96 antibody
      • Ubiquitin-like-conjugating enzyme ATG3 antibody
      see all

    Anti-Apg3 antibody [EPR4801] images

    • All lanes : Anti-Apg3 antibody [EPR4801] (ab108251) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HL-60 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 2000

      Predicted band size : 36 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    • ab108251 staining Apg3 in Mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/200 in 1% BSA in PBS) for 12 hours at 4°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

      See Abreview

    • ab108251, at a 1/100 dilution, staining Human Apg3 in muscle, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
    • Immunofluorescent staining of HeLa cells using ab108251 at 1/100.

    References for Anti-Apg3 antibody [EPR4801] (ab108251)

    This product has been referenced in:
    • Russ DW  et al. Muscle-specificity of age-related changes in markers of autophagy and sphingolipid metabolism. Biogerontology 16:747-59 (2015). WB ; Rat . Read more (PubMed: 26296420) »
    • Haldar AK  et al. The E2-like conjugation enzyme Atg3 promotes binding of IRG and Gbp proteins to Chlamydia- and Toxoplasma-containing vacuoles and host resistance. PLoS One 9:e86684 (2014). WB ; Mouse . Read more (PubMed: 24466199) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 15 µg
    Gel Running Conditions Reduced Denaturing (4-20%)
    Sample Mouse Tissue lysate - whole (Lung)
    Specification Lung
    Treatment 1x10^7 S. aureus peritoneal clot
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Mr. Alan Chang

    Verified customer

    Submitted Mar 20 2014

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample Mouse Tissue sections (uterus)
    Specification uterus
    Permeabilization No
    Fixative Formaldehyde
    Username

    Dr. Varkha Agrawal

    Verified customer

    Submitted Nov 14 2013

    Application Western blot
    Loading amount 5 µg
    Gel Running Conditions Reduced Denaturing (4-12)
    Sample Mouse Tissue lysate - whole (placenta)
    Specification placenta
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Varkha Agrawal

    Verified customer

    Submitted Nov 14 2013

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
    Sample Mouse Tissue sections (Uterus)
    Specification Uterus
    Permeabilization No
    Fixative Paraformaldehyde
    Username

    Dr. Mukesh Jaiswal

    Verified customer

    Submitted Nov 13 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"