Overview

  • Product nameAnti-APG5L/ATG5 antibodySee all APG5L/ATG5 primary antibodies ...
  • Description
    Rabbit polyclonal to APG5L/ATG5
  • Tested applicationsIHC-P, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A synthetic peptide from the internal region of human, mouse and chicken APG5L/ATG5 conjugated to an immunogenic carrier protein

Properties

Applications

Our Abpromise guarantee covers the use of ab64227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P 1/300 - 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/300 - 1/2000.

Target

  • FunctionRequired for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed.
    May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
  • Tissue specificityUbiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
  • Sequence similaritiesBelongs to the ATG5 family.
  • Post-translational
    modifications
    Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
  • Cellular localizationCytoplasm. Colocalizes with nonmuscle actin.
  • Target information above from: UniProt accession Q9H1Y0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • APG 5 antibody
    • APG 5L antibody
    • APG5 antibody
    • APG5 autophagy 5 like antibody
    • APG5 like antibody
    • APG5-like antibody
    • APG5L antibody
    • Apoptosis specific protein antibody
    • Apoptosis-specific protein antibody
    • ASP antibody
    • ATG 5 antibody
    • ATG5 antibody
    • ATG5 autophagy related 5 homolog antibody
    • ATG5_HUMAN antibody
    • Autophagy protein 5 antibody
    • hAPG5 antibody
    • Homolog of S Cerevisiae autophagy 5 antibody
    • OTTHUMP00000040507 antibody
    see all

Anti-APG5L/ATG5 antibody images

  • ab64227 staining mouse Testis tissue sections by immunohistochemistry (Frozen sections). Tissue was fixed in paraformaldehyde and blocked in 1% serum for 10 minutes at   20°C. The primary antibody was diluted 1/800 and incubated with sample for 20 hours at 20°C. The biotin conjugated goat polyclonal to rabbit IgG, diluted 1/500 was used as secondary.

    See Abreview

References for Anti-APG5L/ATG5 antibody (ab64227)

ab64227 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Colon cancer (tissue lysate), U2OS (cell lysate) a)
Loading amount 20 µg
Specification Colon cancer (tissue lysate), U2OS (cell lysate) a
Gel Running Conditions Reduced Denaturing (Pierce precise protein gel 4-20%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Apr 05 2011

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (testis mouse)
Specification testis mouse
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Miss. Silke Vorwald

Verified customer

Submitted Nov 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"