Anti-APG5L/ATG5 antibody [EPR1755(2)] (ab108327)

Overview

• Product nameAnti-APG5L/ATG5 antibody [EPR1755(2)]
  See all APG5L/ATG5 primary antibodies
• Description
  Rabbit monoclonal [EPR1755(2)] to APG5L/ATG5
• Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues in Human APG5L/ATG5.

• Positive control
  • Raji, HeLa, HT-1080, Human fetal kidney, C6, Raw264.7, PC-12 and NIH3T3 cell lysates; Human hepatocellular carcinoma and Human ovarian adenocarcinoma tissue
• General notes

  This product is a recombinant rabbit monoclonal antibody.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

   

  Alternative versions available:

  Anti-APG5L/ATG5 antibody (HRP) [EPR1755(2)] (ab199560)

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
• Storage bufferpH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
• Concentration information loading...
• PurityProtein A purified
• ClonalityMonoclonal
• Clone numberEPR1755(2)
• IsotypeIgG
• Research areas
  • Cell Biology
  • Apoptosis
  • Intracellular
  • Associated Proteins

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ub-like Proteins

  • Cell Biology
  • Apoptosis
  • Phagocytosis

  • Cardiovascular
  • Heart
  • Autophagy
  • APG gene products

  • Cancer
  • Signal transduction
  • Autophagy

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Autophagy and mitophagy

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Autophagy and mitophagy
  • APG gene products

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Cell Death
  • Apoptosis
  • Phagocytosis

  • Cancer
  • Cell Death
  • Apoptosis
  • Metabolism

  • Cancer
  • Cell Death
  • Autophagy
  • Signal Transduction

  • Cancer
  • Cell Death
  • Autophagy
  • APG gene products

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
• Positive Controls
  • NIH 3T3 cytoplasmic extract lysate (ab14873)
  • PC12 cytoplasmic extract lysate (ab14883)
• Related Products
  • Anti-APG5L/ATG5 antibody [EPR1755(2)] (HRP) (ab199560)

Applications

Target

• FunctionInvolved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. The ATG12-ATG5 conjugate also negatively regulates the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Also plays a role in translation or delivery of incoming viral RNA to the translation apparatus. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
  May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.
• Tissue specificityUbiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
• Sequence similaritiesBelongs to the ATG5 family.
• Post-translational
  modificationsConjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
  Acetylated by EP300.
• Cellular localizationCytoplasm. Preautophagosomal structure membrane. Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.

• Target information above from: UniProt accession Q9H1Y0 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 9474 Human
  • Entrez Gene: 11793 Mouse
  • Entrez Gene: 365601 Rat
  • Omim: 604261 Human
  • SwissProt: Q9H1Y0 Human
  • SwissProt: Q99J83 Mouse
  • SwissProt: Q3MQ06 Rat
  • Unigene: 486063 Human

  • Unigene: 22264 Mouse
  • Unigene: 98385 Rat

  see all
• Alternative names
  • APG 5 antibody
  • APG 5L antibody
  • APG5 antibody

  • APG5 autophagy 5 like antibody
  • APG5 like antibody
  • APG5-like antibody
  • APG5L antibody
  • Apoptosis specific protein antibody
  • Apoptosis-specific protein antibody
  • ASP antibody
  • ATG 5 antibody
  • Atg5 antibody
  • ATG5 autophagy related 5 homolog antibody
  • ATG5_HUMAN antibody
  • Autophagy protein 5 antibody
  • Autophagy related 5 antibody
  • hAPG5 antibody
  • Homolog of S Cerevisiae autophagy 5 antibody
  • OTTHUMP00000040507 antibody

  see all