• Product nameAnti-Apg7 antibody
    See all Apg7 primary antibodies
  • Description
    Rabbit polyclonal to Apg7
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH via a C-terminal cysteine residue, corresponding to amino acids 38-50 of Human Apg7

  • Positive control
    • WB:whole extracts of rat PC12 or mouse 3T3 cells. IP: human U87 cell lysates, human brain sections.

Associated products


Our Abpromise guarantee covers the use of ab58735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIP: Use at a concentration of 1 - 2 µg/ml using human U87 cell lysates. 2.5-5.0 mL is
    recommended using biotin/ExtrAvidin Peroxidase staining of heat-retrieved formalin-fixed paraffin embedded tissue from sections of human brain.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionE1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
    • Tissue specificityWidely expressed, especially in kidney, liver, lymph nodes and bone marrow.
    • Sequence similaritiesBelongs to the ATG7 family.
    • DomainThe C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.
      The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.
    • Post-translational
      Acetylated by EP300.
    • Cellular localizationCytoplasm. Preautophagosomal structure. Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1810013K23Rik antibody
      • Apg 7 antibody
      • APG7 autophagy 7 like antibody
      • APG7 autophagy 7-like (S. cerevisiae) antibody
      • APG7 like antibody
      • APG7, S. cerevisiae, homolog of antibody
      • APG7-like antibody
      • APG7L antibody
      • ATG 7 antibody
      • ATG12-activating enzyme E1 ATG7 antibody
      • ATG7 antibody
      • ATG7 autophagy related 7 homolog (S. cerevisiae) antibody
      • ATG7 autophagy related 7 homolog antibody
      • ATG7_HUMAN antibody
      • Atg7l antibody
      • Autophagy 7, S. cerevisiae, homolog of antibody
      • Autophagy related protein 7 antibody
      • Autophagy-related 7 (yeast) antibody
      • Autophagy-related protein 7 antibody
      • DKFZp434N0735 antibody
      • GSA 7 antibody
      • GSA7 antibody
      • hAGP7 antibody
      • Ubiquitin activating enzyme E1 like protein antibody
      • Ubiquitin-activating enzyme E1-like protein antibody
      • Ubiquitin-like modifier-activating enzyme ATG7 antibody
      see all

    Anti-Apg7 antibody images

    • Lanes 1 - 2 : Anti-Apg7 antibody (ab58735) at 1 µg/ml
      Lane 3 : Anti-Apg7 antibody (ab58735) at 2 µg/ml

      Lane 1 : Whole mouse 3T3 cell extract
      Lane 2 : Whole mouse 3T3 cell extract with Apg7 immunizing peptide (human, 38-50)
      Lane 3 : Whole mouse 3T3 cell extract

      HRP conjugated Goat anti-Rabbit IgG
      developed using the ECL technique

      Predicted band size : 78 kDa

    References for Anti-Apg7 antibody (ab58735)

    ab58735 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Tissue lysate - whole (liver)
    Specification liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

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    Submitted Dec 08 2014