The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at a concentration of 1 - 2 µg/ml using human U87 cell lysates. 2.5-5.0 mL is
recommended using biotin/ExtrAvidin Peroxidase staining of heat-retrieved formalin-fixed paraffin embedded tissue from sections of human brain.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionE1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation.
Tissue specificityWidely expressed, especially in kidney, liver, lymph nodes and bone marrow.
Sequence similaritiesBelongs to the ATG7 family.
DomainThe C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12. The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.
Post-translational modificationsAcetylated by EP300.
Cellular localizationCytoplasm. Preautophagosomal structure. Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
Lanes 1 - 2 : Anti-Apg7 antibody (ab58735) at 1 µg/ml Lane 3 : Anti-Apg7 antibody (ab58735) at 2 µg/ml
Lane 1 : Whole mouse 3T3 cell extract Lane 2 : Whole mouse 3T3 cell extract with Apg7 immunizing peptide (human, 38-50) Lane 3 : Whole mouse 3T3 cell extract
Secondary HRP conjugated Goat anti-Rabbit IgG Developed using the ECL technique
Predicted band size : 78 kDa
References for Anti-Apg7 antibody (ab58735)
This product has been referenced in:
Hao Y et al. Lin28a protects against postinfarction myocardial remodeling and dysfunction through Sirt1 activation and autophagy enhancement. Biochem Biophys Res Commun479:833-840 (2016).
Read more (PubMed: 27693787) »