Overview

  • Product nameAnti-APG8A antibody
    See all APG8A primary antibodies
  • Description
    Rabbit polyclonal to APG8A
  • Tested applicationsSuitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Arabidopsis thaliana
    Predicted to work with: Human
  • Immunogen

    Recombinant Arabidopsis thaliana APG8A from E. coli.

Properties

Applications

Our Abpromise guarantee covers the use of ab77003 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 14 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 24463818

Target

  • RelevanceAPG8A is involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. It may mediate the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton.
  • Cellular localizationCytoplasmic vesicle › cvt vesicle membrane; Lipid-anchor. Cytoplasmic vesicle › autophagosome membrane; Lipid-anchor. Vacuole membrane; Lipid-anchor. Note= Membrane-associated through a lipid anchor.
  • Database links
    • Alternative names
      • APG8A (autophagy 8A) antibody
      • AT4G21980 antibody
      • ATG8 antibody
      • ATG8A antibody
      • Autophagy 8A antibody
      • Autophagy related protein 8a antibody
      • Autophagy related ubiquitin like modifier ATG8a antibody
      • F1N20.80 antibody
      • F1N20_80 antibody
      see all

    Anti-APG8A antibody images

    • All lanes : Anti-APG8A antibody (ab77003) at 1/500 dilution

      Lane 1 : Arabidopsis whole plant extract
      Lane 2 : Maize whole plant extract

      Secondary
      HRP-conjugated anti-rabbit IgG. at 1/20000 dilution

      Predicted band size : 14 kDa

    References for Anti-APG8A antibody (ab77003)

    This product has been referenced in:
    • Yoshimoto K  et al. Organ-specific quality control of plant peroxisomes is mediated by autophagy. J Cell Sci 127:1161-8 (2014). IHC-Fr ; Arabidopsis thaliana . Read more (PubMed: 24463818) »
    • Derrien B  et al. Degradation of the antiviral component ARGONAUTE1 by the autophagy pathway. Proc Natl Acad Sci U S A 109:15942-6 (2012). WB . Read more (PubMed: 23019378) »

    See all 3 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Plants Tissue lysate - other (leaves, roots or whole seedlings)
    Gel Running Conditions Non-reduced Denaturing (15% PA running gel)
    Loading amount 60000 µg
    Treatment starvation in presence of 20 micromol L-1 E64 for 48hrs
    Specification leaves, roots or whole seedlings
    Blocking step Blotto B reagent (Santa Cruz Biotech) in TBS as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 4°C
    Username

    Dr. Olga Voitsekhovskaja

    Verified customer

    Submitted Oct 07 2016

    We have not tested this antibody with Barley samples however as this antibody supposed to react with plant samples hence the possibility of its cross reactivity with Barley is quite high.

    Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

    To our knowledge, ab77003 has not been tested inimmunogold EM. Therefore, I can offer a discount off a f...

    Read More

    Thank you very much for contacting us.
    Apparently, Chung and colleagues performed their SDS-PAGE with and without 6 M urea, as reported in their publication (attached). You may find details about this protocol in the Materials and Methods section,...

    Read More

    Thank you for your enquiry. A full length, 6xHis tagged protein was used as the immunogen to create ab77003. I hope this is helpful. Please contact us again if you need any further information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"