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Products:Neuroscience >> Neurology process >> Notch Pathway
Overview
Product name
Anti-Aph 1b antibody
See all Aph 1b products (3) ...
Description
Rabbit polyclonal to Aph 1b
Specificity
This antibody reacts with Aph 1b.
Tested applications
WB, IF, Flow Cyt, Electron Microscopymore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from loop region of human Aph-1b.
Epitope
This antibody recognizes the sequence CKLLKKASEGLKSINPGETAPSMR of human Aph 1b.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.005% Sodium Azide
Constituents: 16.6% Glycerol, PBS, 1mg/ml BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
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Western blot - Aph 1b antibody (ab24614)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IF
Flow Cyt
Electron Microscopy
Application notes
EM: Use at an assay dependent dilution (PMID 19171875).
Flow Cyt: Use at an assay dependent dilution (PMID 19171875).
IF: 1/1000.
WB: 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Probable subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex. Probably present in a minority of gamma-secretase complexes compared to APH1A.
Tissue specificity
Weakly or not expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, colon, skeletal muscle, heart and brain.
Sequence similarities
Belongs to the APH-1 family.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ8WW43
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Anterior pharynx defective 1 homolog B antibody
- Anterior pharynx defective 1B like antibody
- Anterior pharynx defective 1b short splicing variant antibody
see all
Database links
- Entrez Gene: 83464 Human
- Omim: 607630 Human
- SwissProt: Q8WW43 Human
- Unigene: 511703 Human
Anti-Aph 1b antibody images:
Western blot - Aph 1b antibody (ab24614)
Lane 1 : Anti-Aph 1b antibody (ab24614) at 1/1000 dilution
Lane 2 : Anti-Aph 1b antibody (ab24614) at 1/2500 dilution
Lane 1 : 60ug protein
Lane 2 : 60ug protein
Predicted band size : 28 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa (possible dimer).
References for Anti-Aph 1b antibody (ab24614)
This product has been referenced in:
- Radke ALet al. Mature human eosinophils express functional Notch ligands mediating eosinophil autocrine regulation. Blood 113:3092-101 (2009). Electron Microscopy, Flow Cyt; Human.Read more (PubMed: 19171875) »
See 1 publication for this product
Publishing research using ab24614? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"