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Western blot - Aph 1b antibody (ab24614)
(enlarge)
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Product Name
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Aph 1b antibody
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See all Aph 1b antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Aph 1b
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Immunogen
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Synthetic peptide from loop region of human Aph-1b.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody reacts with Aph 1b.
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Epitope
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This antibody recognizes the sequence CKLLKKASEGLKSINPGETAPSMR of human Aph 1b.
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Tested applications
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EM, Flow Cyt, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
EM: Use at an assay dependent dilution (PMID 19171875).
Flow Cyt: Use at an assay dependent dilution (PMID 19171875).
IF: 1/1000.
WB: 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 28 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Integral membrane protein.
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Research areas
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Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurology process >> Notch Pathway
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Relevance
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The gamma secretase is a multiprotein complex consisting of presenilin,nicastrin, Aph-1 and Pen-2; all four proteins are necessary for full proteolytic activity. The gamma secretase complex catalyzes cleavage of a number of integral membrane proteins including Amyloid precursor protein, Notch, Erb4 and E-cadherin. While Aph-1 stabilizes the presenilin holoprotein within the gamma secretase complex, Pen-2 is required for endoproteolysis of presenilin and activation of the gamma secretase. The Aph-1 homologue on human chromosome 1 is termed Aph-1a and on human chromosome 15 is referred to as Aph-1b.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.005% Sodium Azide
Constituents: 16.6% Glycerol, PBS, 1mg/ml BSA Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Aph 1b antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Aph 1b antibody images, references, products related to ab24614 and other tools.
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Aph 1b antibody images:
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 Western blot - Aph 1b antibody (ab24614)
Lane 1 : Aph 1b antibody (ab24614) at 1/1000 dilution
Lane 2 : Aph 1b antibody (ab24614) at 1/2500 dilution
Lane 1 : 60ug protein
Lane 2 : 60ug protein
Predicted band size : 28 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Additional bands at : 40 kDa (possible dimer).
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References for Aph 1b antibody (ab24614)
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This product has been used in:
Radke AL et al. Mature human eosinophils express functional Notch ligands mediating eosinophil autocrine regulation. Blood 113:3092-101 (2009). EM, Flow Cyt; Human. PubMed: 19171875
If you publish research using ab24614 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Aph 1b
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Aph 1b antibody - more information
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Customer reviews (feedback) regarding Aph 1b antibody |
Customer FAQs regarding Aph 1b antibody |
Protocols for Aph 1b antibody |
Price and availability of products related to Aph 1b antibody |
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Aph 1b antibody - related products:
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Aph 1b antibody - other tools:
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Contact Abcam with a Technical Enquiry about Aph 1b antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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