Overview

  • Product nameAnti-APH1a antibody
    See all APH1a primary antibodies
  • Description
    Rabbit polyclonal to APH1a
  • SpecificityRecognises both isoforms of APH1a (encoded on chromosome 1)
  • Tested applicationsSuitable for: IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from residues 50 - 150 of Human APH1a.

    .

  • Positive control
    • This antibody gave a positive signal in human and mouse brain lysates in WB and in mouse brain tissue sections in IF.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab12104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 30 kDa).

Target

  • FunctionEssential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
  • Tissue specificityWidely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.
  • Sequence similaritiesBelongs to the APH-1 family.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6530402N02Rik antibody
    • AL138795.3 antibody
    • Anterior Pharynx Defective 1 antibody
    • Anterior pharynx defective 1 homolog A antibody
    • APH 1A antibody
    • Aph 1alpha antibody
    • APH-1a antibody
    • Aph-1alpha antibody
    • Aph1a antibody
    • APH1A gamma secretase subunit antibody
    • APH1A_HUMAN antibody
    • CGI 78 antibody
    • CGI78 antibody
    • Gamma secretase subunit APH 1A antibody
    • Gamma Secretase Subunit APH1a antibody
    • Gamma-secretase subunit APH-1A antibody
    • Likely ortholog of C. elegans anterior pharynx defective 1A antibody
    • Presenilin Stabilization Factor antibody
    • Presenilin-stabilization factor antibody
    • PSF antibody
    • UNQ579/PRO1141 antibody
    see all

Anti-APH1a antibody images

  • All lanes : Anti-APH1a antibody (ab12104) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 57 kDa,67 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab12104 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • ab12104 staining APH1a in mouse brain tissue section (cortex) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG, diluted 1/1000 was used as secondary. ab12104 produces a very pretty staining in neuronal processes in the cortex and the hippocampus.

    See Abreview

  • All lanes : Anti-APH1a antibody (ab12104) at 1/500 dilution

    Lane 1 : Brain (Human) tissue lysate
    Lane 2 : Brain (Mouse) tissue lysate
    Lane 3 : Brain (Human) tissue lysate with Human APH1a peptide (ab12394) at 1 µg/ml
    Lane 4 : Brain (Mouse) tissue lysate with Human APH1a peptide (ab12394) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 30 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 21 kDa (possible isoform),75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References for Anti-APH1a antibody (ab12104)

This product has been referenced in:
  • Tanveer R  et al. The Endocannabinoid, Anandamide, Augments Notch-1 Signaling in Cultured Cortical Neurons Exposed to Amyloid-ß and in the Cortex of Aged Rats. J Biol Chem 287:34709-21 (2012). WB ; Rat . Read more (PubMed: 22891244) »
  • Donmez G  et al. SIRT1 Suppresses beta-Amyloid Production by Activating the alpha-Secretase Gene ADAM10. Cell 142:320-332 (2010). WB ; Mouse . Read more (PubMed: 20655472) »

See all 2 Publications for this product

Product Wall

I am happy to help. Please let me know if you have any further questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain sections)
Specification Brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Feb 08 2009

We have received the following information from the source of ab16555: This protein is an integral membrane protein (Potential). It is predominantly located in the endoplasmic reticulum and in the cis-Golgi. Tissue specificity: Widely expressed. Ex...

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