The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 29 kDa).
FunctionEssential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Tissue specificityWidely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the same level.
Sequence similaritiesBelongs to the APH-1 family.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
Likely ortholog of C. elegans anterior pharynx defective 1A antibody
Presenilin Stabilization Factor antibody
Presenilin-stabilization factor antibody
Anti-APH1a antibody - Aminoterminal end images
Western blot - APH1a antibody (ab62521)
Lane 1 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 0.5 µg/ml Lane 2 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 1 µg/ml Lane 3 : Anti-APH1a antibody - Aminoterminal end (ab62521) at 2 µg/ml
Lane 1 : RAW264.7 cell lysate Lane 2 : RAW264.7 cell lysate Lane 3 : RAW264.7 cell lysate