Overview

  • Product nameAnti-APLF antibody
    See all APLF primary antibodies
  • Description
    Rabbit polyclonal to APLF
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic non-phosphopeptide derived from human APLF around the phosphorylation site of serine 116 (R-N-SP-Q-V).

  • Positive control
    • Human brain tissue; HT-29 cell extracts.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ELISA 1/40000.
IHC-P 1/50 - 1/100.

Target

  • FunctionNuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.
  • Sequence similaritiesBelongs to the APLF family.
    Contains 1 FHA-like domain.
    Contains 2 PBZ-type zinc fingers.
  • DomainThe PBZ-type zinc fingers (also named CYR) mediate non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and promotes its recruitment to DNA damage sites.
    The FHA-like domain mediates interaction with XRCC1 and XRCC4.
  • Post-translational
    modifications
    Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc fingers, the protein is also covalently poly-ADP-ribosylated by PARP1.
    Phosphorylated in an ATM-dependent manner upon double-strand DNA break.
  • Cellular localizationNucleus. Cytoplasm > cytosol. Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2010301N04Rik antibody
    • AI452191 antibody
    • Aplf antibody
    • APLF_HUMAN antibody
    • Aprataxin and PNK-like factor antibody
    • Apurinic-apyrimidinic endonuclease APLF antibody
    • C2orf13 antibody
    • PNK and APTX like FHA protein antibody
    • PNK and APTX-like FHA domain-containing protein antibody
    • RGD1565557 antibody
    • XIP1 antibody
    • XRCC1 interacting protein 1 antibody
    • XRCC1-interacting protein 1 antibody
    see all

Anti-APLF antibody images

  • ab79371 at 1/50 dilution staining APLF in human brain by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-APLF antibody (ab79371) at 1/500 dilution

    Lane 1 : HT-29 cell extracts
    Lane 2 : HT-29 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 57 kDa

References for Anti-APLF antibody (ab79371)

ab79371 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"