Overview

  • Product name
  • Description
    Rabbit polyclonal to APLP1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human APLP1.

  • Positive control
    • This antibody gave a positive signal in Human Brain Tissue Lysate. This antibody gave a positive result when used in the following methanol fixed cell lines:SKNSH.

Properties

Applications

Our Abpromise guarantee covers the use of ab94957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.
    The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.
  • Tissue specificity
    Expressed in the cerebral cortex where it is localized to the postsynaptic density (PSD).
  • Sequence similarities
    Belongs to the APP family.
  • Domain
    The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain. However, additional amino acids either N- or C-terminal to the NPXY motif are often required for complete interaction. The NPXY site is also involved in clathrin-mediated endocytosis.
  • Post-translational
    modifications
    Proteolytically cleaved by caspases during neuronal apoptosis. Cleaved, in vitro, at Asp-620 by caspase-3.
    N- and O-glycosylated. O-glycosylation with core 1 or possibly core 8 glycans. Glycosylation on Ser-227 is the preferred site to Thr-228.
  • Cellular localization
    Cell membrane and Cytoplasm. C-terminally processed in the Golgi complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMYLOID BETA A4 PRECURSOR-LIKE PROTEIN 1 antibody
    • AMYLOID PRECURSOR-LIKE PROTEIN antibody
    • Amyloid-like protein 1 precursor antibody
    • APLP 1 antibody
    • APLP antibody
    • APLP-1 antibody
    • Aplp1 antibody
    • APLP1_HUMAN antibody
    • C30 antibody
    see all

Images

  • Anti-APLP1 antibody (ab94957) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ab94957 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab94957 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab94957 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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