Overview

  • Product name
    Anti-APLP1 antibody - N-terminal
    See all APLP1 primary antibodies
  • Description
    Rabbit polyclonal to APLP1 - N-terminal
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human APLP1 aa 101-120 (N terminal).
    Sequence:

    ELQIARVEQATQAIPMERWC


    Database link: P51693

  • Positive control
    • Human Lung Cancer Tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab191066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 72 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.
    The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.
  • Tissue specificity
    Expressed in the cerebral cortex where it is localized to the postsynaptic density (PSD).
  • Sequence similarities
    Belongs to the APP family.
  • Domain
    The NPXY sequence motif found in many tyrosine-phosphorylated proteins is required for the specific binding of the PID domain. However, additional amino acids either N- or C-terminal to the NPXY motif are often required for complete interaction. The NPXY site is also involved in clathrin-mediated endocytosis.
  • Post-translational
    modifications
    Proteolytically cleaved by caspases during neuronal apoptosis. Cleaved, in vitro, at Asp-620 by caspase-3.
    N- and O-glycosylated. O-glycosylation with core 1 or possibly core 8 glycans. Glycosylation on Ser-227 is the preferred site to Thr-228.
  • Cellular localization
    Cell membrane and Cytoplasm. C-terminally processed in the Golgi complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMYLOID BETA A4 PRECURSOR-LIKE PROTEIN 1 antibody
    • AMYLOID PRECURSOR-LIKE PROTEIN antibody
    • Amyloid-like protein 1 precursor antibody
    • APLP 1 antibody
    • APLP antibody
    • APLP-1 antibody
    • Aplp1 antibody
    • APLP1_HUMAN antibody
    • C30 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human lung cancer tissue labelling APLP1 with ab191066 antibody at a concentration of 1 µg/ml.

References

ab191066 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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