Overview

  • Product name
  • Description
    Rabbit polyclonal to APMAP
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide within N terminal amino acids 72-121 (FKEPPLLLGV LHPNTKLRQA ERLFENQLVG PESIAHIGDV MFTGTADGRV) of Human APMAP (NP_065392)

  • Positive control
    • Fetal liver lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

Target

  • Function
    Exhibits strong arylesterase activity with beta-naphthyl acetate and phenyl acetate. May play a role in adipocyte differentiation.
  • Tissue specificity
    Liver, glomerular and tubular structures of the kidney, endothelial cells, arterial wall and pancreatic islets of Langerhans (at protein level). Found ubiquitously in adult as well as in embryonic tissues. In adult tissue, the highest expression is found in the liver, placenta and heart. Found on the cell surface of monocytes. In embryonic tissue, the highest expression levels is found in the liver and the kidney.
  • Sequence similarities
    Belongs to the strictosidine synthase family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adipocyte plasma membrane associated protein antibody
    • Adipocyte plasma membrane-associated protein antibody
    • apmap antibody
    • APMAP_HUMAN antibody
    • BSCv antibody
    • BSCv protein antibody
    • C20orf3 antibody
    • Chromosome 20 open reading frame 3 antibody
    • Protein BSCv antibody
    see all

Anti-APMAP antibody images

  • Anti-APMAP antibody (ab81176) at 1 µg/ml + Fetal liver lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 46 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-APMAP antibody (ab81176)

ab81176 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver lysate)
Gel Running Conditions
Reduced Denaturing (NUPAGE 4-12% BT GEL 1.5MM)
Loading amount
20 µg
Specification
Liver lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Jul 04 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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