Anti-APOA1BP antibody (ab75114)
Key features and details
- Rabbit polyclonal to APOA1BP
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-APOA1BP antibody
See all APOA1BP primary antibodies -
Description
Rabbit polyclonal to APOA1BP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Extracts from HeLa cells. A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 32 kDa.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/500 - 1/1000. |
Target
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Relevance
APOA1BP interacts with apolipoprotein A-I (apoA-I), the major apolipoprotein of high-density lipoproteins (HDLs). It is secreted into some bodily fluids, and its synthesis and secretion are stimulated in vitro by incubating cells with apoA-I. -
Cellular localization
Mitochondrion. Secreted. Note: In sperm, secretion gradually increases during capacitation -
Database links
- Entrez Gene: 128240 Human
- Entrez Gene: 246703 Mouse
- Entrez Gene: 295229 Rat
- Omim: 608862 Human
- SwissProt: Q8NCW5 Human
- SwissProt: Q8K4Z3 Mouse
- SwissProt: B0BNM1 Rat
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Alternative names
- AI BP antibody
- AIBP antibody
- ApoA I binding protein antibody
see all
Images
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All lanes : Anti-APOA1BP antibody (ab75114) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from HeLa cells with immunizing peptide at 5 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 32 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of APOA1BP in A549 cells, using ab75114, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab75114 has been referenced in 2 publications.
- Duan M et al. Mitochondrial apolipoprotein A-I binding protein alleviates atherosclerosis by regulating mitophagy and macrophage polarization. Cell Commun Signal 20:60 (2022). PubMed: 35525979
- Choi SH et al. AIBP augments cholesterol efflux from alveolar macrophages to surfactant and reduces acute lung inflammation. JCI Insight 3:N/A (2018). PubMed: 30135304