The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesPeptide ELISA: Antibody detection limit dilution 1/1000.
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 32 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceAPOA1BP interacts with apolipoprotein A-I (apoA-I), the major apolipoprotein of high-density lipoproteins (HDLs). It is secreted into some bodily fluids, and its synthesis and secretion are stimulated in vitro by incubating cells with apoA-I.
Cellular localizationMitochondrion. Secreted. Note: In sperm, secretion gradually increases during capacitation