Overview

  • Product name
  • Description
    Mouse polyclonal to APOA4
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-396 of Human APOA4 (AAH74764.1).

  • Positive control
    • APOA4 transfected 293T cell line lysate, Human small Intestine tissue

Applications

Our Abpromise guarantee covers the use of ab167350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Apo AIV antibody
    • APOA 4 antibody
    • ApoA IV antibody
    • APOA4 antibody
    • Apolipoprotein A IV antibody
    • Apolipoprotein A4 antibody
    • MGC142154 antibody
    • MGC142156 antibody
    see all

Images

  • All lanes : Anti-APOA4 antibody (ab167350) at 1/500 dilution

    Lane 1 : APOA4 transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 15 µl per lane.

    Predicted band size: 45 kDa

  • Immunohistochemical analysis of paraffin embedded Human small Intestine tissue labeling APOA4 with ab167350 antibody at 3 µg/ml.

References

ab167350 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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