Overview

  • Product name
  • Description
    Goat polyclonal to APOA4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey
  • Immunogen

    Synthetic peptide corresponding to Human APOA4 aa 359-371 (internal sequence).
    Sequence:

    C-KEKESQDKTLSLP


    (Peptide available as ab78150)

  • Positive control
    • WB: Human duodenum tissue lysate. IHC-P: Human intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab59036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with Human APOA4 peptide (ab78150).
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties
  • Cellular localization
    Secreted
  • Database links
  • Alternative names
    • Apo AIV antibody
    • APOA 4 antibody
    • ApoA IV antibody
    • APOA4 antibody
    • Apolipoprotein A IV antibody
    • Apolipoprotein A4 antibody
    • MGC142154 antibody
    • MGC142156 antibody
    see all

Images

  • Anti-APOA4 antibody (ab59036) at 0.01 µg/ml + Human Duodenum lysate (35µg protein in RIPA buffer)

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human intestine tissue, staining APOA4 with ab59036 at 2 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).

References

ab59036 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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