Overview

  • Product nameAnti-APOA4 antibody
    See all APOA4 primary antibodies
  • Description
    Goat polyclonal to APOA4
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide corresponding to Human APOA4 aa 359-371 (internal sequence).
    Sequence:

    C-KEKESQDKTLSLP


    (Peptide available as ab78150)

  • Positive control
    • WB: Human duodenum tissue lysate. IHC-P: Human intestine tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab59036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).Can be blocked with Human APOA4 peptide (ab78150).
IHC-P Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceAPOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Apo AIV antibody
    • APOA 4 antibody
    • ApoA IV antibody
    • APOA4 antibody
    • Apolipoprotein A IV antibody
    • Apolipoprotein A4 antibody
    • MGC142154 antibody
    • MGC142156 antibody
    see all

Anti-APOA4 antibody images

  • Anti-APOA4 antibody (ab59036) at 0.01 µg/ml + Human Duodenum lysate (35µg protein in RIPA buffer)

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human intestine tissue, staining APOA4 with ab59036 at 2 µg/ml. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6).

References for Anti-APOA4 antibody (ab59036)

ab59036 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab59036.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"