Recombinant
RabMAb

Anti-APOA4 antibody [EPR2895] (ab108200)

Overview

  • Product name
    Anti-APOA4 antibody [EPR2895]
    See all APOA4 primary antibodies
  • Description
    Rabbit monoclonal [EPR2895] to APOA4
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human APOA4.

  • Positive control
    • Human plasma
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Applications

Our Abpromise guarantee covers the use of ab108200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Relevance
      APOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • Apo AIV antibody
      • APOA 4 antibody
      • ApoA IV antibody
      • APOA4 antibody
      • Apolipoprotein A IV antibody
      • Apolipoprotein A4 antibody
      • MGC142154 antibody
      • MGC142156 antibody
      see all

    Images

    • Anti-APOA4 antibody [EPR2895] (ab108200) at 1/1000 dilution + Human plasma at 10 µg

      Predicted band size : 45 kDa
    • Western blot analysis of APOA4 in immunoprecipitation pellets from Human plasma (Lane 1) or 1XPBS (negative control) (Lane 2) using ab108200 at 1/10 dilution for IP and at 1/1000 dilution for WB detection.

    References

    This product has been referenced in:
    • Cubedo J  et al. ApoL1 levels in high density lipoprotein and cardiovascular event presentation in patients with familial hypercholesterolemia. J Lipid Res 57:1059-73 (2016). Read more (PubMed: 27112635) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Serum (human plasma)
    Loading amount
    0.5 µg
    Specification
    human plasma
    Gel Running Conditions
    Reduced Denaturing (9%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Dr. Hua Jiang

    Verified customer

    Submitted Aug 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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