Anti-APOA4 antibody [EPR2895] (ab108200)

Overview

  • Product nameAnti-APOA4 antibody [EPR2895]
    See all APOA4 primary antibodies
  • Description
    Rabbit monoclonal [EPR2895] to APOA4
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human APOA4.

  • Positive control
    • Human plasma lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Applications

Our Abpromise guarantee covers the use of ab108200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 45 kDa).
IP 1/10 - 1/100.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • RelevanceAPOA4 (apolipoprotein A-IV) is a component of HDL and chylomicrons. Its primary site of synthesis is the intestine, in association with lymph chylomicron particles. Although its precise function is not known, APOA4 is a potent activator of lecithin-cholesterol acyltransferase (LCAT) in vitro. In rodents, Apo A-IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti-inflammatory and antiatherogenic properties
    • Cellular localizationSecreted
    • Database links
    • Alternative names
      • Apo AIV antibody
      • APOA 4 antibody
      • ApoA IV antibody
      • APOA4 antibody
      • Apolipoprotein A IV antibody
      • Apolipoprotein A4 antibody
      • MGC142154 antibody
      • MGC142156 antibody
      see all

    Anti-APOA4 antibody [EPR2895] images

    • Anti-APOA4 antibody [EPR2895] (ab108200) at 1/1000 dilution + Human plasma cell lysate at 10 µg

      Predicted band size : 45 kDa
    • Western blot analysis of APOA4 in immunoprecipitation pellets from Human plasma lysate (Lane 1) or 1XPBS (negative control) (Lane 2) using ab108200 at 1/10 dilution for IP and at 1/1000 dilution for WB detection.

    References for Anti-APOA4 antibody [EPR2895] (ab108200)

    ab108200 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Serum (human plasma)
    Loading amount 0.5 µg
    Specification human plasma
    Gel Running Conditions Reduced Denaturing (9%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Dr. Hua Jiang

    Verified customer

    Submitted Aug 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"