Overview

  • Product name
  • Description
    Goat polyclonal to APOBEC3D
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    C-PKRQSNHRQEVYFR

    , corresponding to internal sequence amino acids 61-74 of Human APOBEC3D (NP_689639.2).

  • Positive control
    • A549 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab115021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    Unknown. Unable to reduce HIV-1 infectivity in vitro.
  • Sequence similarities
    Belongs to the cytidine and deoxycytidylate deaminase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • A3D antibody
    • ABC3D_HUMAN antibody
    • APOBEC3D antibody
    • APOBEC3DE antibody
    • APOBEC3E antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3D antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3E pseudogene antibody
    • ARP6 antibody
    • bK150C2.9 antibody
    • BK150C2.9 protein antibody
    • DNA dC >dU editing enzyme APOBEC 3D antibody
    • DNA dC->dU-editing enzyme APOBEC-3D antibody
    • hCG_41704 antibody
    • HCG41704, isoform CRA_b antibody
    • Probable DNA dC->dU-editing enzyme APOBEC-3D antibody
    see all

Anti-APOBEC3D antibody images

  • Anti-APOBEC3D antibody (ab115021) at 0.3 µg/ml + A549 lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References for Anti-APOBEC3D antibody (ab115021)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Thank you for your response and for attaching the images.
This is to let you know that as you requested I have placed a new order for you - for one vial of ab105869 as a free of charge replacement (alternative for ab115021) and the new order numbe...

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Thank you very much for your updates. Your time and effort would be very much appreciated
I wonder if you could send a WB image so that I can take a look at the different band size(s)? It may well that endogenously-expressed and transfected protei...

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Thank you for your response and your comments.
Northern blot analysis detected APOBEC3D expression in colorectal adenocarcinoma cells, chronic myelogenous leukemia cells, lung carcinoma and HeLa cells. No expression was detected in normal tissues....

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Thank you for your enquiry regarding ab115021 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
I would like to reassure you that our Abpromise appli...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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