Anti-APOBEC3D + APOBEC3F antibody (ab74205)

Overview

  • Product nameAnti-APOBEC3D + APOBEC3F antibody
  • Description
    Rabbit polyclonal to APOBEC3D + APOBEC3F
  • SpecificityDetects both APOBEC3D + APOBEC3F
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the internal region of human APOBEC3D + APOBEC3F

  • Positive control
    • Human placenta tissue; extracts from MCF7 or COS7 cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab74205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 42 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

Target

  • RelevanceAPOBEC3D + APOBEC3F both belong to the cytidine and deoxycytidylate deaminase family. After being packaged into HIV-1 virions, APOBEC3F blocks productive infection by massively editing dC residues to dU on the DNA minus strand during reverse transcription. The editing of the minus strand DNA of HIV-1 during reverse transcription leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. There are two named isoforms.
  • Database links
  • Alternative names
    • APOBEC3D antibody
    • APOBEC3E antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3F antibody
    • Apolipoprotein B mRNA editing enzyme, catalytic polypeptide like 3D antibody
    • ARP6 antibody
    • ARP8 antibody
    • Induced upon T cell activation antibody
    • KA6 antibody
    see all

Anti-APOBEC3D + APOBEC3F antibody images

  • Immunohistochemistry analysis of paraffin-embedded human placenta tissue using 1/50 ab74205.
    Left image: no pre-treatment.
    Right image: pre-treatment with immunizing peptide.
  • All lanes : Anti-APOBEC3D + APOBEC3F antibody (ab74205) at 1/500 dilution

    Lane 1 : Extracts from MCF-7 cells
    Lane 2 : Extracts from COS-7 cells
    Lane 3 : Extracts from COS-7 cells plus 5µg immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-APOBEC3D + APOBEC3F antibody (ab74205)

ab74205 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab74205.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"