The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 42 kDa.
1/50 - 1/100.
RelevanceAPOBEC3D + APOBEC3F both belong to the cytidine and deoxycytidylate deaminase family.
After being packaged into HIV-1 virions, APOBEC3F blocks productive infection by massively editing dC residues to dU on the DNA minus strand during reverse transcription. The editing of the minus strand DNA of HIV-1 during reverse transcription leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. There are two named isoforms.