The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceAPOBEC3F is a human protein with natural antiviral activity, especially against HIV. As such, it is currently the subject of intense scientific investigation, and may lead to the development of new therapeutics useful for treating HIV/AIDS.
After being packaged into HIV-1 virions, APOBEC3F blocks productive infection by massively editing dC residues to dU on the DNA minus strand during reverse transcription. The editing of the minus strand DNA of HIV-1 during reverse transcription leads to G- to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations.