|
|
|
|
Immunocytochemistry/ Immunofluorescence - APOBEC3G antibody (ab54257)
(enlarge)
|
|
|
|
|
Product Name
|
APOBEC3G antibody
|
| |
See all APOBEC3G antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to APOBEC3G
|
|
Immunogen
|
Synthetic peptide: CPFQPWDGLDEHSQDLSGRLRAILQNQEN, corresponding to amino acids 356-384 of Human APOBEC3G
BLAST 'CPFQPWDGLDEHSQDLSGRLRAILQNQEN' with  or 
|
|
Reacts with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ICC/IF, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: 1/250.
WB: 1/500. Predicted molecular weight: 46 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Mainly cytoplasmic. Small amounts are found in the nucleus. During HIV1 infection, virion encapsidated in absence of HIV1 VIF.
|
|
Research areas
|
Immunology >> Immune System Diseases >> Antiviral Signaling
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Deamination
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Microbiology >> Interspecies Interaction >> Host Virus Interaction
|
|
Relevance
|
APOBEC3G is a DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV1 infection) by triggering G-to-A hypermutation in the newly synthetized viral DNA. The replacements C-to-U in the minus strand DNA of HIV1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing.
APOBEC3G is involved in hepatitis B virus (HBV) resistance probably primarily by inhibiting viral pregenomic RNA packaging. This activity is independent of the cytidine deaminase activity, as the inactivated protein is able to inhibit HBV replication. During HBV infection, APOBEC3G does not seem to be involved in the editing of HBV DNA, but this might occur in particular cellular contexts or with specific viral strains.
APOBEC3G is a homodimer. It interacts with APOBEC3B, APOBEC3F and HIV1 VIF in a species specific manner. It is expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines. Normally not expressed in the liver, but it is possible that it is induced in the course of HBV infection, for instance under the influence of cytokines.
APOBEC3G is ubiquitinated in the presence of HIV1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin dependent proteasome pathway.
APOBEC3G inhibits also the infectivity of retroviral particles only distantly related to HIV1, such as the simian immunodeficiency virus (SIV), the equine infectious anemia virus (EIAV) and the murine leukemia virus (MLV).
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Whole antiserum
|
|
Storage buffer
|
Preservative: None
Constituents: 50% Glycerol Material safety datasheet (MSDS) for this product:
Glycerol MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Concentration not determined - why is this?
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this APOBEC3G antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for APOBEC3G antibody images, references, products related to ab54257 and other tools.
|
| |
APOBEC3G antibody images:
|
 Immunocytochemistry/ Immunofluorescence - APOBEC3G antibody (ab54257)
HeLa cell monolayers were transfected with an APOBEC3G expression vector, fixed after 24 hr and hybridised with the anti-APOBEC3G ab54257 (1/500 dilution). Bound antibody was detected using an Alexa Fluor 594-conjugated goat secondary antibody and imaged by confocal microscopy. APOBEC3G is seen (in green) distributed throughout the cytosol, and concentrated in small areas called P-bodies.
|
| |
References for APOBEC3G antibody (ab54257)
|
|
This product has been used in:
Gallois-Montbrun S et al. Antiviral protein APOBEC3G localizes to ribonucleoprotein complexes found in P bodies and stress granules. J Virol 81:2165-78 (2007). PubMed: 17166910
If you publish research using ab54257 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to APOBEC3G
|
| |
APOBEC3G antibody - more information
|
Customer reviews (feedback) regarding APOBEC3G antibody |
Customer FAQs regarding APOBEC3G antibody |
Protocols for APOBEC3G antibody |
Price and availability of products related to APOBEC3G antibody |
| |
APOBEC3G antibody - related products:
|
|
|
| |
APOBEC3G antibody - other tools:
|
Contact Abcam with a Technical Enquiry about APOBEC3G antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
