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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-APOBEC3G antibody
See all APOBEC3G products (6) ...
Description
Rabbit polyclonal to APOBEC3G
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: CPFQPWDGLDEHSQDLSGRLRAILQNQEN, corresponding to amino acids 356-384 of Human APOBEC3G
CPFQPWDGLD EHSQDLSGRL RAILQNQEN
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Immunology >> Immune System Diseases >> Antiviral Signaling
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Deamination
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab54257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 42 kDa (predicted molecular weight: 46 kDa).
ICC/IF: 1/250
Target
Function
DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing.
Tissue specificity
Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines.
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family.
Post-translational
modifications
Ubiquitinated in the presence of HIV-1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway.
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF.
Target information above from: UniProt accessionQ9HC16
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ABC3G_HUMAN antibody
- APOBEC related cytidine deaminase antibody
- APOBEC related protein antibody
see all
Database links
- Entrez Gene: 200316 Human
- Entrez Gene: 60489 Human
- Omim: 607113 Human
- SwissProt: Q9HC16 Human
- Unigene: 659809 Human
- Unigene: 660143 Human
Anti-APOBEC3G antibody images:
Immunocytochemistry/ Immunofluorescence - APOBEC3G antibody (ab54257)
HeLa cell monolayers were transfected with an APOBEC3G expression vector, fixed after 24 hr and hybridised with the anti-APOBEC3G ab54257 (1/500 dilution). Bound antibody was detected using an Alexa Fluor 594-conjugated goat secondary antibody and imaged by confocal microscopy. APOBEC3G is seen (in green) distributed throughout the cytosol, and concentrated in small areas called P-bodies.
Western blot - APOBEC3G antibody (ab54257)
All lanes : Anti-APOBEC3G antibody (ab54257) at 1/500 dilution
Lane 1 : Spleen (Human) Tissue Lysate - adult normal tissue (ab29699)
Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)
Lane 3 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 46 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Additional bands at : 36 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
References for Anti-APOBEC3G antibody (ab54257)
This product has been referenced in:
- Gallois-Montbrun Set al. Antiviral protein APOBEC3G localizes to ribonucleoprotein complexes found in P bodies and stress granules. J Virol 81:2165-78 (2007).Read more (PubMed: 17166910) »
See 1 publication for this product
Publishing research using ab54257? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"