Overview

  • Product nameAnti-APOBEC3G antibody - C-terminal
    See all APOBEC3G primary antibodies
  • Description
    Rabbit polyclonal to APOBEC3G - C-terminal
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human APOBEC3G (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • A375 cell lysate and human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

Target

  • FunctionDNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription, leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF), that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs, but does not display detectable APOB, NF1 and NAT1 mRNA editing.
  • Tissue specificityExpressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines; no expression detected in permissive lymphoid and non-lymphoid cell lines.
  • Sequence similaritiesBelongs to the cytidine and deoxycytidylate deaminase family.
  • Post-translational
    modifications
    Ubiquitinated in the presence of HIV-1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway.
  • Cellular localizationCytoplasm. Nucleus. Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection, virion-encapsidated in absence of HIV-1 VIF.
  • Information by UniProt
  • Database links
  • Alternative names
    • A3G antibody
    • ABC3G_HUMAN antibody
    • APOBEC related cytidine deaminase antibody
    • APOBEC related protein antibody
    • APOBEC-related cytidine deaminase antibody
    • APOBEC-related protein 9 antibody
    • APOBEC-related protein antibody
    • APOBEC3G antibody
    • Apolipoprotein B editing enzyme catalytic polypeptide like 3G antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide 3G antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide like 3G antibody
    • Apolipoprotein B mRNA editing enzyme catalytic subunit 3G antibody
    • apolipoprotein B mRNA editing enzyme cytidine deaminase antibody
    • apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like antibody
    • ARCD antibody
    • ARP-9 antibody
    • ARP9 antibody
    • bK150C2.7 antibody
    • CEM-15 antibody
    • CEM15 antibody
    • deoxycytidine deaminase antibody
    • dJ494G10.1 antibody
    • DNA dC dU editing enzyme APOBEC 3G antibody
    • DNA dC->dU editing enzyme antibody
    • DNA dC->dU-editing enzyme APOBEC-3G antibody
    • EC 3.5.4. antibody
    • FLJ12740 antibody
    • MDS019 antibody
    • OTTHUMP00000028911 antibody
    • phorbolin-like protein antibody
    • phorbolin-like protein MDS019 antibody
    see all

Anti-APOBEC3G antibody - C-terminal images

  • Anti-APOBEC3G antibody - C-terminal (ab71634) at 1/100 dilution + A375 cell lysate at 12.5 µg

    Predicted band size : 46 kDa
    Observed band size : 46 kDa
    Additional bands at : 33 kDa,35 kDa. We are unsure as to the identity of these extra bands.
  • ab71634 at 1/50 dilution, staining APOBEC3G in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

References for Anti-APOBEC3G antibody - C-terminal (ab71634)

This product has been referenced in:
  • Pereira NZ  et al. Upregulation of innate antiviral restricting factor expression in the cord blood and decidual tissue of HIV-infected mothers. PLoS One 8:e84917 (2013). WB ; Human . Read more (PubMed: 24367701) »

See 1 Publication for this product

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Thank you for your enquiry. I have searched our catalog for antibodies to APOBEC3G and HIV tat proteins that have been used successfully in ELISA. Here are some recommendations: For APOBEC3G: ab71634 and ab38604, which are both available for €360.00 + ...

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