Overview

  • Product name
    Apocynin
  • Description
    Selective NADPH-oxidase inhibitor
  • Biological description
    Selective NADPH-oxidase inhibitor (IC50 = 10 μM). Inhibits production of reactive oxygen species. Also elicits a range of in vitro and in vivo anti-inflammatory effects.
  • Purity
    > 99%

Properties

Applications

Our Abpromise guarantee covers the use of ab120615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab19534 staining glutathione in A549 cells treated with apocynin (ab120615), by ICC/IF. Increase in glutathione expression correlates with increased concentration of apocynin, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120615 (apocynin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19534 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody.

References

This product has been referenced in:
  • Genovese T  et al. Modulation of NADPH oxidase activation in cerebral ischemia/reperfusion injury in rats. Brain Res 1372:92-102 (2011). Read more (PubMed: 21138737) »
  • Impellizzeri D  et al. Effect of apocynin, a NADPH oxidase inhibitor, on acute lung inflammation. Biochem Pharmacol 81:636-48 (2011). Read more (PubMed: 21147071) »
  • Impellizzeri D  et al. Effect of Apocynin, an inhibitor of NADPH oxidase, in the inflammatory process induced by an experimental model of spinal cord injury. Free Radic Res 45:221-36 (2011). Read more (PubMed: 20954833) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you very much for your call today and for your question about ab120615.

I've looked through some literature and references, and apocynin does seem to reversibly inhibitNADPH oxidase:

http://www.hindawi.com/journals/mi/2008/106...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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