Recombinant
RabMAb

Anti-ApoER2 antibody [EPR3326] (ab108208)

Overview

  • Product name
    Anti-ApoER2 antibody [EPR3326]
    See all ApoER2 primary antibodies
  • Description
    Rabbit monoclonal [EPR3326] to ApoER2
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human ApoER2 aa 950 to the C-terminus (C terminal).
    Database link: Q14114
    (Peptide available as ab193569)

  • Positive control
    • SH-SY5Y, C6, Neuro-2a, Human fetal brain, Mouse brain, and Rat brain lysates,
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 106 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      Cell surface receptor for Reelin (RELN) and apolipoprotein E (apoE)-containing ligands. LRP8 participates in transmitting the extracellular Reelin signal to intracellular signaling processes, by binding to DAB1 on its cytoplasmic tail. Reelin acts via both the VLDL receptor (VLDLR) and LRP8 to regulate DAB1 tyrosine phosphorylation and microtubule function in neurons. LRP8 has higher affinity for Reelin than VLDLR. LRP8 is thus a key component of the Reelin pathway which governs neuronal layering of the forebrain during embryonic brain development. Binds the endoplasmic reticulum resident receptor-associated protein (RAP). Binds dimers of beta 2-glycoprotein I and may be involved in the suppression of platelet aggregation in the vasculature. Highly expressed in the initial segment of the epididymis, where it affects the functional expression of clusterin and phospholipid hydroperoxide glutathione peroxidase (PHGPx), two proteins required for sperm maturation. May also function as an endocytic receptor.
    • Tissue specificity
      Expressed mainly in brain and placenta. Also expressed in platelets and megakaryocytic cells. Not expressed in the liver.
    • Involvement in disease
      Defects in LRP8 are a cause of myocardial infarction type 1 (MCI1) [MIM:608446]. A condition defined by the irreversible necrosis of heart muscle secondary to prolonged ischemia.
    • Sequence similarities
      Belongs to the LDLR family.
      Contains 2 EGF-like domains.
      Contains 7 LDL-receptor class A domains.
      Contains 5 LDL-receptor class B repeats.
    • Domain
      The cytoplasmic domain is involved in the binding of DAB1 and in the recruitment of JNK-interacting proteins. Isoforms, which lack part of the cytoplasmic domain, are unable to recruit members of the family of JNK interacting proteins (JIP) to the cytoplasmic tail.
    • Post-translational
      modifications
      O-glycosylated. Some alternatively spliced isoforms lack the O-linked sugar domain.
      Undergoes sequential, furin and gamma-secretase dependent, proteolytic processing, resulting in the extracellular release of the entire ligand-binding domain as a soluble polypeptide and in the intracellular domain (ICD) release into the cytoplasm. The gamma-secretase-dependent proteolytical processing occurs after the bulk of the extracellular domain has been shed, in a furin-dependent manner, in alternatively spliced isoforms carrying the furin cleavage site. Hypoglycosylation (mainly hypo-O-glycosylation) leads to increased extracellular cleavage, which in turn results in accelerating release of the intracellular domain (ICD) by the gamma-secretase. The resulting receptor fragment is able to inhibit Reelin signaling and in particular the Reelin-induced DAB1 phosphorylation.
      Tyrosine phosphorylated upon apoE binding.
      Ubiquitinated by MYLIP leading to degradation.
    • Cellular localization
      Cell membrane. Secreted. Isoforms that contain the exon coding for a furin-type cleavage site are proteolytically processed, leading to a secreted receptor fragment.
    • Information by UniProt
    • Database links
    • Alternative names
      • APOER2 antibody
      • Apolipoprotein E receptor 2 antibody
      • low density lipoprotein receptor-related protein 8 antibody
      • Low-density lipoprotein receptor-related protein 8 antibody
      • LRP-8 antibody
      • LRP8 antibody
      • LRP8_HUMAN antibody
      see all

    Anti-ApoER2 antibody [EPR3326] images

    • All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/10000 dilution (purified)

      Lane 1 : SH-SY5Y (human neuroblastoma) whole cell lysate
      Lane 2 : Human fetal brain tissue lysate
      Lane 3 : Mouse brain tissue lysate
      Lane 4 : C6 (rat glioma) whole cell lysate
      Lane 5 : Neuro-2a (mouse neuroblastoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size : 106 kDa
      Observed band size : 130-170 kDa (why is the actual band size different from the predicted?)

      Blocking and diluting buffer 5% NFDM/TBST.

      130kDa: Immature form;

      170kDa: Glycosylated mature form. (PMID:24344333, 24532792, 24705369 and 25233900)

    • All lanes : Anti-ApoER2 antibody [EPR3326] (ab108208) at 1/1000 dilution (unpurified)

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : Neuro-2a cell lysate
      Lane 4 : Human Fetal Brain cell lysate
      Lane 5 : Mouse Fetal Brain cell lysate
      Lane 6 : Rat Fetal Brain cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 106 kDa
    • ab108208 (purified) at 1/40 immunoprecipitating ApoER2 in SH-SY5Y (human neuroblastoma) whole cell lysate.

      Lane 1 (input): SH-SY5Y whole cell lysate 10ug

      Lane 2 (+): ab108208 + SH-SY5Y whole cell lysate

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108208 in SH-SY5Y whole cell lysate

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References for Anti-ApoER2 antibody [EPR3326] (ab108208)

    This product has been referenced in:
    • Hinrich AJ  et al. Therapeutic correction of ApoER2 splicing in Alzheimer's disease mice using antisense oligonucleotides. EMBO Mol Med 8:328-45 (2016). WB ; Mouse . Read more (PubMed: 26902204) »
    • Telese F  et al. LRP8-Reelin-Regulated Neuronal Enhancer Signature Underlying Learning and Memory Formation. Neuron 86:696-710 (2015). Read more (PubMed: 25892301) »

    See all 7 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Human THP-1 cell line, various mouse tissues)
    Loading amount
    15 µg
    Specification
    Human THP-1 cell line, various mouse tissues
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gel (Novex))
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

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    Verified customer

    Submitted Nov 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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