The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceAPOLD1 is an endothelial cell early response protein that may be involved in angiogenesis. It may play a role in activity dependent changes of brain vasculature. APOLD1 may affect blood brain permeability.
Cellular localizationCell membrane; Multi pass membrane protein