Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:2,000.
WB: Preliminary experiments gave an approximate 37 kDa band in human brain (frontal cortex) lysates after 1 µg/ml antibody staining. The 37 kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from researchers who use this antibody, such as successful results being obtained with other lysates.
RelevanceAPOLD1 is an endothelial cell early response protein that may be involved in angiogenesis. It may play a role in activity dependent changes of brain vasculature. APOLD1 may affect blood brain permeability.
Cellular localizationCell membrane; Multi pass membrane protein