Apolipoprotein AI (APOA1) Human ELISA Kit (ab108803)

Overview

  • Product nameApolipoprotein AI (APOA1) Human ELISA Kit
    See all Apolipoprotein A I kits
  • Detection methodColorimetric
  • Precision
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Cell culture extracts, Cerebral Spinal Fluid
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 3 ng/ml
  • Range
    3.13 ng/ml - 200 ng/ml
  • Recovery

    95 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Apolipoprotein AI (APOA1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AI concentrations in urine, saliva, milk, CSF and cell culture samples.

    An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AI captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionParticipates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
  • Tissue specificityMajor protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.
  • Involvement in diseaseHigh density lipoprotein deficiency 2
    High density lipoprotein deficiency 1
    APOA1 mutations may be involved in the pathogenesis of amyloid polyneuropathy-nephropathy Iowa type, also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III (PubMed:3142462 and PubMed:2123470). The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis.
    Amyloidosis 8
  • Sequence similaritiesBelongs to the apolipoprotein A1/A4/E family.
  • Post-translational
    modifications
    Palmitoylated.
    Met-110 and Met-136 are oxidized to methionine sulfoxides.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Alternative names
    • Apo-AI
    • ApoA-I
    • APOA1
    • APOA1_HUMAN
    • Apolipoprotein A-I
    • Apolipoprotein A-I(1-242)
    • Apolipoprotein A1
    • Truncated apolipoprotein A-I
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Apolipoprotein AI (APOA1) Human ELISA Kit images

  • Representative Standard Curve using ab108803

Protocols

References for Apolipoprotein AI (APOA1) Human ELISA Kit (ab108803)

ab108803 has not yet been referenced specifically in any publications.

Product Wall


The amount of standard for lot GR132237-1 = 3.2 ug (micrograms).

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I can confirm that the main difference between these kits is that the ab108804 Apolipoprotein A1 Human ELISA Kit uses a competitive ELISA quantification, whereas ab108803 Apolipoprotein A I Human ELISA Kituses sta...

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