Apolipoprotein AI (APOA1) Human ELISA Kit (ab108803)

Overview

  • Product nameApolipoprotein AI (APOA1) Human ELISA Kit
    See all Apolipoprotein A I kits
  • Detection methodColorimetric
  • Precision
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Cell culture extracts, Cerebral Spinal Fluid
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 3 ng/ml
  • Range
    3.13 ng/ml - 200 ng/ml
  • Recovery

    95 %

  • Assay time
    4h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Apolipoprotein AI (APOA1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of apolipoprotein AI concentrations in urine, saliva, milk, CSF and cell culture samples.

    An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein AI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein AI captured in plate.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Apolipoprotein AI (APOA1) Human ELISA Kit images

  • Representative Standard Curve using ab108803

Protocols

References for Apolipoprotein AI (APOA1) Human ELISA Kit (ab108803)

ab108803 has not yet been referenced specifically in any publications.

Product Wall


The amount of standard for lot GR132237-1 = 3.2 ug (micrograms).

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I can confirm that the main difference between these kits is that the ab108804 Apolipoprotein A1 Human ELISA Kit uses a competitive ELISA quantification, whereas ab108803 Apolipoprotein A I Human ELISA Kituses sta...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"