Apolipoprotein AI (APOA1) Human ELISA Kit (ab108804)

Overview

  • Product nameApolipoprotein AI (APOA1) Human ELISA KitSee all Apolipoprotein A I kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 7.2%
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    1 µg/ml
  • Range
    0.625 µg/ml - 20 µg/ml
  • Recovery

    98 %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Apolipoprotein AI Human (APOA1) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein AI levels in plasma and serum.

    An Apolipoprotein AI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Apolipoprotein AI is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Apolipoprotein AI captured in plate.

  • Tested applicationsCompetitive ELISA more details
  • PlatformMicroplate

Properties

  • Cellular localizationCell Membrane
  • Alternative names
    • Apo AI
    • Apo-AI
    • Apo-AI
    • ApoA I
    • ApoA-I
    • ApoA-I
    • APOA1
    • APOA1/APOC3 fusion gene
    • APOA1_HUMAN
    • Apolipoprotein A I precursor
    • Apolipoprotein A-I
    • Apolipoprotein A-I(1-242)
    • Apolipoprotein A-I(1-242)
    • Apolipoprotein A1
    • Apolipoprotein A1
    • Apolipoprotein AI
    • Apolipoprotein of high density lipoprotein
    • ApolipoproteinAI
    • Brp14
    • Ltw1
    • Lvtw1
    • MGC117399
    • Sep1
    • Sep2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Competitive ELISA Use at an assay dependent concentration.

Apolipoprotein AI (APOA1) Human ELISA Kit images

  • Representative Standard Curve using ab108804

Protocols

References for Apolipoprotein AI (APOA1) Human ELISA Kit (ab108804)

ab108804 has not yet been referenced specifically in any publications.

Product Wall

Only the assays for APOB (ab108807) and APOE (ab108813) have been tested with CSF. They are suitable for this kind of sample. The APOA1 assays have not been tested with CSF yet, but there are plans to test ab108803. Kit ab108804, the competitive ELISA ...

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Vielen Dank für Ihre Anfrage.


Leider haben wir kein weiteres Kit der Charge GR104136 mehr in unserem Lager (weltweit).

Diese Kits ist normalerweise sehr zuverlässig, aber Sie haben Recht, es gibt eine Komponente, die ni...

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Thank you for your patience with this reply.

I heard back from the lab that for the kits below, the antibodies were raised against recombinant proteins. The epitopes have not been mapped.

1) Apolipoprotein A1 Human ELISA Kit
2)...

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Abreview for Apolipoprotein A1 Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum (Human plasma)
Specification: Human plasma
Amount of Sample: 2 µg
Type of Kit Used: ELISA
Positive control: recombinant apolipoprotein A1
Did you use the Abcam Kit protocol?: Yes
Username

Abcam user community

Verified customer

Submitted Jan 15 2013


Below is the information for the standard specific to your lot:

Standard Curve: Reconstitute the 120 mg of Apo A-I Standard with 3 ml of Diluent to generate a solution of 40 mg/ml. Allow the standard to sit for 10 minutes with gentle ag...

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I contacted the laboratory, and they responded that the Apo A-1 standard should be in micrograms, not nanograms. The current lot's standard is delivered at 60ug and 3mL of diluent would make the 20ug/ml solution. If you respond with your specific ...

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Vielen Dank für Ihre Anfrage. Ich entschuldige mich für die Wartezeit, ich musste auf nähere Angaben aus dem produzierenden Labor warten.

Ich freue mich Ihnen nun bestätigen zu können, dass beide Standards aus aufgere...

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Thank you for contacting Abcam.

After receiving the customer's complaint and abreview showing cross-reactivity with apoB using the kit ab108804 in May, we retested the kit for cross-reactivity. There was no significant cross-reactivity with ot...

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Here is the shelf life information you requested:
ab108804 - 6 months if kit is unused and reagents stored at 4 deg C or -20 deg C as appropriate; 1 month after opening and limiting reagent is microtiter plate storage
ab108807 - 6 months...

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Thank you for your inquiry.
I am assuming that you are planning to use ab108804? http://www.abcam.com/index.html?datasheet=108804 http://www.abcam.com/index.html?datasheet=108804.
I can confirm that ab108804 has not been tested with cell cult...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"