Anti-Apolipoprotein B antibody [12G10] (ab63960)


  • Product name
    Anti-Apolipoprotein B antibody [12G10]
    See all Apolipoprotein B primary antibodies
  • Description
    Mouse monoclonal [12G10] to Apolipoprotein B
  • Specificity
    ab63960 reacts with LDL of human plasma. It is specific to the T4 fragment of Apo-B (residues 1-1297). No crossreactions with other human plasma proteins were detected.
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified human LDL

  • Positive control
    • This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7.


Our Abpromise guarantee covers the use of ab63960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 515 kDa.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • Involvement in disease
    Hypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • Sequence similarities
    Contains 1 vitellogenin domain.
  • Post-translational
    Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • Cellular localization
    Cytoplasm. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo B-100 antibody
    • Apo B-48 antibody
    • ApoB 100 antibody
    • ApoB 48 antibody
    • APOB antibody
    • APOB_HUMAN antibody
    • Apolipoprotein B (including Ag(x) antigen) antibody
    • Apolipoprotein B 100 antibody
    • Apolipoprotein B antibody
    • Apolipoprotein B-48 antibody
    • Apolipoprotein B48 antibody
    • FLDB antibody
    • LDLCQ4 antibody
    see all

Anti-Apolipoprotein B antibody [12G10] images

  • ab63960 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63960 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (100%) HeLa and HepG2 cells, and also in formaldehyde fixed (4%) HeLa, HepG2, and MCF-7 cells at 5ug/ml.

References for Anti-Apolipoprotein B antibody [12G10] (ab63960)

ab63960 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I am very pleased to hear you would like to make use of our AbTrial offer and test ab63960 in Mouse cells with ICC/IF.

Expiration date: 30th March 2013

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Thank you for contacting us.

Unfortunately ab63960 has not been tested in any applications other than WB and Flow Cytometry. It doesn’t mean it won’t work in other applications, but we don’t have data to support it.
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