Anti-Apolipoprotein B antibody [7B8] (ab39560)

Overview

  • Product nameAnti-Apolipoprotein B antibody [7B8]
    See all Apolipoprotein B primary antibodies
  • Description
    Mouse monoclonal [7B8] to Apolipoprotein B
  • SpecificityAb39560 has specificity for Apolipoprotein B from serum/plasma. No crossreactivity is seen with Apolipoprotein A1.
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human LDL.

Applications

Our Abpromise guarantee covers the use of ab39560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Ab39560 reacts with Apolipoprotein B from human serum/plasma, when used in sandwich ELISA in combination with a polyclonal antibody against Apolipoprotein B, and also with isolated LDL directly coated onto the microtiter plate.
WB Use at an assay dependent concentration. Predicted molecular weight: 260 kDa. Ab39560 binds to Apolipoprotein B in reduced as well as in non-reduced forms, using isolated LDL as antigen.
IHC-P 1/100 - 1/400.

Target

  • FunctionApolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • Involvement in diseaseHypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • Sequence similaritiesContains 1 vitellogenin domain.
  • Post-translational
    modifications
    Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • Cellular localizationCytoplasm. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo B-100 antibody
    • Apo B-48 antibody
    • ApoB 100 antibody
    • ApoB 48 antibody
    • APOB antibody
    • APOB_HUMAN antibody
    • Apolipoprotein B 100 antibody
    • Apolipoprotein B antibody
    • Apolipoprotein B-48 antibody
    • Apolipoprotein B48 antibody
    • FLDB antibody
    • LDLCQ4 antibody
    see all

Anti-Apolipoprotein B antibody [7B8] images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Apolipoprotein B with ab39560 at a dilution of 1/100. Signal is strong in the veins of the glomerulus.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Apolipoprotein B with ab39560 at a dilution of 1/400. Plasma of the veins was stained strongly.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Apolipoprotein B with ab39560 at a dilution of 1/400. Plasma veins were stained strongly.

References for Anti-Apolipoprotein B antibody [7B8] (ab39560)

ab39560 has not yet been referenced specifically in any publications.

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I now heard back form the lab and they confirmed that was never experimentally tested if ab39560 also binds to apoB48 alone.

As stated on the datasheet ab39560 reacts with Apolipoprotein B from human ser...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"