Overview

  • Product nameAnti-Apolipoprotein B antibody
    See all Apolipoprotein B primary antibodies
  • Description
    Rabbit polyclonal to Apolipoprotein B
  • SpecificityCross-reacts with Apo B48 and B100.
  • Tested applicationsSuitable for: IHC-Fr, IP, ELISA, IHC-P, Immunodiffusion, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length native protein (purified) corresponding to Mouse Apolipoprotein B. Apolipoprotein B48 purified from pooled mouse plasma very low-density lipoprotein.

Applications

Our Abpromise guarantee covers the use of ab20737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000.
IP Use at an assay dependent concentration. PubMed: 18497885
ELISA Use at an assay dependent concentration. PubMed: 21668821
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunodiffusion Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 549 kDa.

Target

  • FunctionApolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
  • Involvement in diseaseHypobetalipoproteinemia, familial, 1
    Familial ligand-defective apolipoprotein B-100
    Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
  • Sequence similaritiesContains 1 vitellogenin domain.
  • Post-translational
    modifications
    Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
  • Cellular localizationCytoplasm. Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo B-100 antibody
    • Apo B-48 antibody
    • ApoB 100 antibody
    • ApoB 48 antibody
    • APOB antibody
    • APOB_HUMAN antibody
    • Apolipoprotein B 100 antibody
    • Apolipoprotein B antibody
    • Apolipoprotein B-48 antibody
    • Apolipoprotein B48 antibody
    • FLDB antibody
    • LDLCQ4 antibody
    see all

Anti-Apolipoprotein B antibody images

  • ab20737 staining Apoplipoprotein B in murine intestinal tissue by Immunohistochemistry (frozen sections). Tissue was fixed with 10% formalin and permeabilized using Triton X-100. Samples were then blocked with 10% serum for 30 minutes at 24°C followed by incubation with the primary antibody, at a 1/100 dilution, for 16 hours at 4°C. A donkey anti-rabbit F(ab')2 fragment conjugated to Cy5® was used as secondary antibody at a 1/1000 dilution.

    See Abreview

  • ab20737 at a 1/100 dilution staining Apolipoprotein B in murine small intestinal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed. Samples were then permeabilized using 0.2% PBST and blocked using 20% serum. An Alexa Fluor® 488 conjugated Goat anti-rabbit IgG was used as the secondary at a 1/100 dilution.

References for Anti-Apolipoprotein B antibody (ab20737)

This product has been referenced in:
  • Ye R  et al. Adiponectin is essential for lipid homeostasis and survival under insulin deficiency and promotes ß-cell regeneration. Elife 3:N/A (2014). IHC ; Mouse . Read more (PubMed: 25339419) »
  • Etxebarria A  et al. Advantages and versatility of fluorescence-based methodology to characterize the functionality of LDLR and class mutation assignment. PLoS One 9:e112677 (2014). Read more (PubMed: 25386756) »

See all 11 Publications for this product

Product Wall


Below is information regarding the use of ab20737 in western blot.

1. Working dilution for immunoblotting - start low 1:100 - 1:500.
2. Molecular Weight of Apo B100 is 500kDa; Apo B48 is 250kDa. The western blot was run against purifi...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Kidney)
Specification Kidney
Fixative 10% neutral buffered formalin
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6.0 (20 min, 93*C)
Permeabilization No
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 21°C
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Verified customer

Submitted Jan 24 2013

Because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will w...

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Thank you for your inquiry.

I am happy to confirm that we offer an Anti-Apolipoprotein B antibody that is tested and guaranteed for ICC and WB in rat, human and mouse samples:

ab117317b (Anti-Apolipoprotein B antibody)

htt...

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Thank you for contacting us. Your credit note ID is xxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice ...

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Thank you for your reply.

I can gladly create this credit note/refund for you provided that you have purchase this item within the last six months. In order for me to issue this could you please provide me the Abcam order confirmation number...

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Thank you for your reply. If your Abcam order was placed by credit card, the credit note indicates the dollar amount that will be refunded to your credit card account. Please allow 2-5 business days for the credit to process and appear on your state...

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Thank you for taking time to provide this information and for contacting us. I am sorry to hear this antibody is not providing satisfactory results and that these suggestions did not help.

The details provided will enable us to investigate...

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Thank you for contacting us.

The expected molecular weight of this protein is 549KDa. As recommended by our http://www.abcam.com/index.html?pageconfig=resource&rid=11403#B1 the bigger the size of the protein of interest, the lower the per...

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Thank you for contacting us regarding ab20737.

It was a pleasure to speak with you today, I do hope that the dilution suggestion will help when using this product in ELISA.Please do not hesitate to contact us if you need any more advice or...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"