The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Radial Immunodiffusion (RID): 1/10 - 1/25.
WB: Use at an assay dependent dilution. Predicted molecular weight: 516 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Involvement in disease
Hypobetalipoproteinemia, familial, 1 Familial ligand-defective apolipoprotein B-100 Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
Contains 1 vitellogenin domain.
Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.