The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesRadial Immunodiffusion (RID): 1/10 - 1/25.
WB: Use at an assay dependent dilution. Predicted molecular weight: 516 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionApolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
Involvement in diseaseHypobetalipoproteinemia, familial, 1 Familial ligand-defective apolipoprotein B-100 Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
References for Anti-Apolipoprotein B antibody (ab31992)
This product has been referenced in:
Johnson LA et al. Diabetic atherosclerosis in APOE*4 mice: synergy between lipoprotein metabolism and vascular inflammation. J Lipid Res54:386-96 (2013).
Read more (PubMed: 23204275) »
Maczuga P et al. Embedding siRNA sequences targeting Apolipoprotein B100 in shRNA and miRNA scaffolds results in differential processing and in vivo efficacy. Mol Ther : (2012).
Read more (PubMed: 23089734) »