Overview

  • Product name
    Anti-Apolipoprotein B antibody
    See all Apolipoprotein B primary antibodies
  • Description
    Rabbit polyclonal to Apolipoprotein B
  • Specificity
    ab31992 recognizes Apolipoprotein B48 and B100, as these proteins are highly conserved.
  • Tested applications
    Suitable for: WB, Immunodiffusionmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    SDS PAGE purified mouse Apolipoprotein B48 (very low density lipoprotein) from pooled mouse plasma.

Applications

Our Abpromise guarantee covers the use of ab31992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Immunodiffusion
  • Application notes
    Radial Immunodiffusion (RID): 1/10 - 1/25.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 516 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
    • Involvement in disease
      Hypobetalipoproteinemia, familial, 1
      Familial ligand-defective apolipoprotein B-100
      Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.
    • Sequence similarities
      Contains 1 vitellogenin domain.
    • Post-translational
      modifications
      Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
    • Cellular localization
      Cytoplasm. Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Apo B-100 antibody
      • Apo B-48 antibody
      • ApoB 100 antibody
      • ApoB 48 antibody
      • APOB antibody
      • APOB_HUMAN antibody
      • Apolipoprotein B (including Ag(x) antigen) antibody
      • Apolipoprotein B 100 antibody
      • Apolipoprotein B antibody
      • Apolipoprotein B-48 antibody
      • Apolipoprotein B48 antibody
      • FLDB antibody
      • LDLCQ4 antibody
      see all

    References for Anti-Apolipoprotein B antibody (ab31992)

    This product has been referenced in:
    • Johnson LA  et al. Diabetic atherosclerosis in APOE*4 mice: synergy between lipoprotein metabolism and vascular inflammation. J Lipid Res 54:386-96 (2013). Mouse . Read more (PubMed: 23204275) »
    • Maczuga P  et al. Embedding siRNA sequences targeting Apolipoprotein B100 in shRNA and miRNA scaffolds results in differential processing and in vivo efficacy. Mol Ther : (2012). Read more (PubMed: 23089734) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver, brain)
    Loading amount
    15 µg
    Specification
    liver, brain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    5%milk+1%BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 11 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (Mouse C57/BL6 EDTA plasma)
    Loading amount
    30 µg
    Specification
    Mouse C57/BL6 EDTA plasma
    Treatment
    starving O/N
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Dr. Andreas Gille

    Verified customer

    Submitted Feb 12 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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